| Autor: |
Jun JV; Department of Chemistry , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States., Haney CM; Department of Chemistry , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States., Karpowicz RJ Jr; Department of Pathology and Laboratory Medicine , Center for Neurodegenerative Disease Research, University of Pennsylvania , 3600 Spruce Street , Philadelphia , Pennsylvania 19104 , United States., Giannakoulias S; Department of Chemistry , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States., Lee VM; Department of Pathology and Laboratory Medicine , Center for Neurodegenerative Disease Research, University of Pennsylvania , 3600 Spruce Street , Philadelphia , Pennsylvania 19104 , United States., Petersson EJ; Department of Chemistry , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States., Chenoweth DM; Department of Chemistry , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States. |
| Abstrakt: |
Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azide-alkyne cycloaddition ("click" reaction). To demonstrate its utility, we have applied CPX to study (1) trafficking of biologically relevant synthetic vesicles and (2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules. |
