Autor: |
Gapon MN, Telesmanitch NR, Ternovskaya LN, Chaika SO, Chaika IA, Mikashinovitch ZI, Tverdokhlebova TI |
Jazyk: |
ruština |
Zdroj: |
Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2016 Jun; Vol. 61 (6), pp. 371-5. |
Abstrakt: |
The time-of-flight mass spectrometer analysis of freshly separated human colibacillus populations for identification of markers of proteom specific for hemolytic and non-hemolytic strains, Escherichia-associant of opportunistic enterobacteria. The material consisted of 20 strains of mono-population of hemolytic Escherichia; 100 strains of mono-population of non-hemolytic Escherichia; 15 non-hemolytic strains isolated from association with opportunistic enterobacteria. The protein profiling was implemented on mass spectrometer MALDI-TOF MS Autoflex “Bruker Daltonik”. The value within type of peak mass-charge with 100% intensity equal to 9000 Da can be used as a differentiating indication of detection of hemolytic Escherichia at mass-spectrometer biotyping. The proteom characteristics of strains of various populations within Escherichia coli species are given. The taxon-specific markers for proteom differentiation of population of Escherichia are given too. |
Databáze: |
MEDLINE |
Externí odkaz: |
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