Clinical and histopathologic prognostic implications of the expression of cytokeratins 8, 10, 13, 14, 16, 18 and 19 in oral and oropharyngeal squamous cell carcinoma.

Autor: Safadi RA; Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan; In sabbatical leave to College of Dentistry, King Saud Bin Abdul Aziz University for Health Sciences Riyadh, Saudi Arabia. Electronic address: rsafadi@just.edu.jo., Abdullah NI; King Hussein Cancer Center, Amman, Jordan., Alaaraj RF; King Hussein Cancer Center, Amman, Jordan., Bader DH; Ministry of Health, Amman, Jordan., Divakar DD; College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia., Hamasha AA; Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan; In sabbatical leave to College of Dentistry, King Saud Bin Abdul Aziz University for Health Sciences Riyadh, Saudi Arabia., Sughayer MA; King Hussein Cancer Center, Amman, Jordan.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2019 Mar; Vol. 99, pp. 1-8. Date of Electronic Publication: 2018 Dec 16.
DOI: 10.1016/j.archoralbio.2018.12.007
Abstrakt: Objectives: To identify cytokeratins (CK) of significant correlations with clinical and histopathologic prognostic parameters in oral and oropharyngeal squamous cell carcinoma (SCC).
Design: The sample consisted of 100 cases retrieved from the archives of the Pathology Department/ King Hussein Cancer Center/Amman/ Jordan. Recorded data included: age, gender, location, grade, depth of invasion, the presence of epithelial dysplasia, tumor size, lymph node metastasis, number of positive lymph nodes, distant metastases, clinical stage, local recurrence, treatment modalities and 5-year survival rate. Immunohistochemical staining of 7 cytokeratins: 8, 10, 13, 14, 16, 18, and 19 was performed using standard protocols. Stained sections were digitized and analyzed using ImageJ-color deconvolution to identify the percentage of cytokeratin-positive area (score). Statistical tests used were: student t-test, analysis of variance, bivariate analysis and logistic regression.
Results: Lower CK8,18, 19 scores correlated with lower 5-year survival rate. Higher CK19 and lower CK 10, 14, 16 scores were associated with distant metastasis. Increased CK8, 18, 19 scores correlated with higher stage and with higher depth of invasion. Increased CK18 scores correlated with increased local recurrence. Higher CK10, 13, 16 scores correlated with well-differentiated grade. Higher CK19 and lower CK16 scores were associated with adjacent epithelial dysplasia. Regression analysis showed that better 5-year survival rate was significantly correlated with increased CK16, decreased CK18 and 19 scores.
Conclusion: Expression scores of a panel of cytokeratin are potential prognostic indicators for 5-year survival and correlates with other prognostic parameters.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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