Oxidative capacity and hemolytic activity of settled dust from moisture-damaged schools.

Autor: Huttunen K; Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland.; School of Biosciences, Cardiff University, Cardiff, UK.; Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health (HICE), Neuherberg, Germany., Wlodarczyk AJ; School of Biosciences, Cardiff University, Cardiff, UK.; Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health (HICE), Neuherberg, Germany., Tirkkonen J; Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland., Mikkonen S; Department of Applied Physics, University of Eastern Finland, Kuopio, Finland., Täubel M; Environmental Health Unit, Department of Health Security, National Institute for Health and Welfare, Kuopio, Finland., Krop E; Division Environmental Epidemiology, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands., Jacobs J; Division Environmental Epidemiology, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands., Pekkanen J; Environmental Health Unit, Department of Health Security, National Institute for Health and Welfare, Kuopio, Finland.; Department of Public Health, University of Helsinki, Helsinki, Finland., Heederik D; Division Environmental Epidemiology, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands., Zock JP; ISGlobal, Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain.; Universitat Pompeu Fabra (UPF), Barcelona, Spain.; CIBER Epidemiología y Salud Pública (CIBERESP), Madrid, Spain., Hyvärinen A; Environmental Health Unit, Department of Health Security, National Institute for Health and Welfare, Kuopio, Finland., Hirvonen MR; Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland.; Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health (HICE), Neuherberg, Germany., Adams R; Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK., Jones T; School of Earth & Ocean Sciences, Cardiff University, Cardiff, UK., Zimmermann R; Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health (HICE), Neuherberg, Germany.; Joint Mass Spectrometry Centre, Institute of Chemistry, Analytical Chemistry, University of Rostock, Rostock, Germany., BéruBé K; School of Biosciences, Cardiff University, Cardiff, UK.; Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health (HICE), Neuherberg, Germany.; Cardiff Institute for Tissue Engineering & Repair (CITER), Cardiff University, Cardiff, Wales, UK.
Jazyk: angličtina
Zdroj: Indoor air [Indoor Air] 2019 Mar; Vol. 29 (2), pp. 299-307. Date of Electronic Publication: 2019 Jan 21.
DOI: 10.1111/ina.12527
Abstrakt: Exposure to moisture-damaged indoor environments is associated with adverse respiratory health effects, but responsible factors remain unidentified. In order to explore possible mechanisms behind these effects, the oxidative capacity and hemolytic activity of settled dust samples (n = 25) collected from moisture-damaged and non-damaged schools in Spain, the Netherlands, and Finland were evaluated and matched against the microbial content of the sample. Oxidative capacity was determined with plasmid scission assay and hemolytic activity by assessing the damage to isolated human red blood cells. The microbial content of the samples was measured with quantitative PCR assays for selected microbial groups and by analyzing the cell wall markers ergosterol, muramic acid, endotoxins, and glucans. The moisture observations in the schools were associated with some of the microbial components in the dust, and microbial determinants grouped together increased the oxidative capacity. Oxidative capacity was also affected by particle concentration and country of origin. Two out of 14 studied dust samples from moisture-damaged schools demonstrated some hemolytic activity. The results indicate that the microbial component connected with moisture damage is associated with increased oxidative stress and that hemolysis should be studied further as one possible mechanism contributing to the adverse health effects of moisture-damaged buildings.
(© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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