Amputation-induced reactive oxygen species signaling is required for axolotl tail regeneration.

Autor: Al Haj Baddar NW; Department of Biology, University of Kentucky, Lexington, Kentucky.; Department of Neuroscience and Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, Kentucky., Chithrala A; Paul Laurence Dunbar High School, Lexington, Kentucky., Voss SR; Department of Neuroscience and Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, Kentucky.; Ambystoma Genetic Stock Center, University of Kentucky, Lexington, Kentucky.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2019 Feb; Vol. 248 (2), pp. 189-196. Date of Electronic Publication: 2018 Dec 21.
DOI: 10.1002/dvdy.5
Abstrakt: Background: Among vertebrates, salamanders are unparalleled in their ability to regenerate appendages throughput life. However, little is known about early signals that initiate regeneration in salamanders.
Results: Ambystoma mexicanum embryos were administered tail amputations to investigate the timing of reactive oxygen species (ROS) production and the requirement of ROS for regeneration. ROS detected by dihydroethidium increased within minutes of axolotl tail amputation and levels remained high for 24 hr. Pharmacological inhibition of ROS producing enzymes with diphenyleneiodonium chloride (DPI) and VAS2870 reduced ROS levels. Furthermore, DPI treatment reduced cellular proliferation and inhibited tail outgrowth.
Conclusions: The results show that ROS levels increase in response to injury and are required for tail regeneration. These findings suggest that ROS provide instructive, if not initiating cues, for salamander tail regeneration. Developmental Dynamics 248:189-196, 2019. © 2018 Wiley Periodicals, Inc.
(© 2018 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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