| Autor: |
Tanwar V; 1 Dorothy M. Davis Heart and Lung Research Institute College of Medicine The Ohio State University Columbus OH.; 2 College of Nursing The Ohio State University Columbus OH., Adelstein JM; 1 Dorothy M. Davis Heart and Lung Research Institute College of Medicine The Ohio State University Columbus OH.; 2 College of Nursing The Ohio State University Columbus OH., Grimmer JA; 1 Dorothy M. Davis Heart and Lung Research Institute College of Medicine The Ohio State University Columbus OH.; 2 College of Nursing The Ohio State University Columbus OH., Youtz DJ; 1 Dorothy M. Davis Heart and Lung Research Institute College of Medicine The Ohio State University Columbus OH.; 2 College of Nursing The Ohio State University Columbus OH., Katapadi A; 3 Medical Student Research Program The Ohio State University College of Medicine Columbus OH., Sugar BP; 3 Medical Student Research Program The Ohio State University College of Medicine Columbus OH., Falvo MJ; 5 Department of Veterans Affairs War Related Illness and Injury Study Center New Jersey Health Care System East Orange NJ., Baer LA; 4 Department of Physiology and Cell Biology The Ohio State University College of Medicine Columbus OH., Stanford KI; 1 Dorothy M. Davis Heart and Lung Research Institute College of Medicine The Ohio State University Columbus OH.; 4 Department of Physiology and Cell Biology The Ohio State University College of Medicine Columbus OH., Wold LE; 1 Dorothy M. Davis Heart and Lung Research Institute College of Medicine The Ohio State University Columbus OH.; 2 College of Nursing The Ohio State University Columbus OH.; 4 Department of Physiology and Cell Biology The Ohio State University College of Medicine Columbus OH. |
| Abstrakt: |
Background Particulate matter (particles < 2.5 μm [ PM 2.5 ]) exposure during the in utero and postnatal developmental periods causes cardiac dysfunction during adulthood. Here, we investigated the potential priming effects of preconception exposure of PM 2.5 on cardiac function in adult offspring. Methods and Results Male and female friend leukemia virus b (FVB) mice were exposed to either filtered air ( FA ) or PM 2.5 at an average concentration of 38.58 μg/m 3 for 6 hours/day, 5 days/week for 3 months. Mice were then crossbred into 2 groups: (1) FA male × FA female (both parents were exposed to FA preconception) and, (2) PM 2.5male × PM 2.5female (both parents were exposed to PM 2.5 preconception). Male offspring were divided: (1) preconception FA (offspring born to FA exposed parents) and, (2) preconception PM 2.5 (offspring born to PM 2.5 exposed parents) and analyzed at 3 months of age. Echocardiography identified increased left ventricular end systolic volume and reduced posterior wall thickness, reduced %fractional shortening and %ejection fraction in preconception PM 2.5 offspring. Cardiomyocytes isolated from preconception PM 2.5 offspring showed reduced %peak shortening, -dL/dT, TPS 90 and slower calcium reuptake (tau). Gene and protein expression revealed modifications in markers of inflammation ( IL -6, IL -15, TNF α, NF қB, CRP , CD 26E, CD 26P, intercellular adhesion molecule 1, and monocyte chemoattractant protein-1) profibrosis (collagen type III alpha 1 chain), oxidative stress ( NOS 2), antioxidants (Nrf2, SOD , catalase), Ca 2+ regulatory proteins ( SERCA 2a, p- PLN , NCX ), and epigenetic regulators (Dnmt1, Dnmt3a, Dnmt3b, Sirt1, and Sirt2) in preconception PM 2.5 offspring. Conclusions Preconception exposure to PM 2.5 results in global cardiac dysfunction in adult offspring, suggesting that abnormalities during development are not limited to the prenatal or postnatal periods but can also be determined before conception. |
Plný text