First report of two consecutive respiratory syncytial virus outbreaks by the novel genotypes ON-1 and NA-2 in a neonatal intensive care unit.

Autor:	Silva DGBPD; Santa Casa de São Paulo, Departamento de Pediatria, São Paulo, SP, Brazil., Almeida FJ; Santa Casa de São Paulo, Unidade de Infectologia Pediátrica, São Paulo, SP, Brazil., Arnoni MV; Santa Casa de São Paulo, Unidade de Controle de Infecção Hospitalar, São Paulo, SP, Brazil., Sáfadi MAP; Santa Casa de São Paulo, Unidade de Infectologia Pediátrica, São Paulo, SP, Brazil., Mimica MJ; Santa Casa de São Paulo, Unidade de Infectologia Pediátrica, São Paulo, SP, Brazil., Jarovsky D; Santa Casa de São Paulo, Unidade de Infectologia Pediátrica, São Paulo, SP, Brazil. Electronic address: daniel@jarovsky.com.br., Rossetti GPA; Santa Casa de São Paulo, Unidade Neonatal, São Paulo, SP, Brazil., Magalhães M; Santa Casa de São Paulo, Unidade Neonatal, São Paulo, SP, Brazil., Oliveira DBL; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil., Thomazelli LM; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil., Colmanetti TC; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil., Durigon EL; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil., Berezin EN; Santa Casa de São Paulo, Unidade de Infectologia Pediátrica, São Paulo, SP, Brazil.
Jazyk:	angličtina
Zdroj:	Jornal de pediatria [J Pediatr (Rio J)] 2020 Mar - Apr; Vol. 96 (2), pp. 233-239. Date of Electronic Publication: 2018 Dec 12.
DOI:	10.1016/j.jped.2018.10.014
Abstrakt:	Objective: Respiratory syncytial virus is a pathogen frequently involved in nosocomial outbreaks. Although several studies have reported nosocomial outbreaks in neonatal intensive care units, molecular epidemiology data are scarce. Here, the authors describe two consecutive respiratory syncytial virus outbreaks caused by genotypes ON-1 and NA-2 in a neonatal intensive care unit in São Paulo, Brazil. Methods: A prospective search for respiratory syncytial virus was performed after diagnosing the index case and four other symptomatic newborns in the neonatal intensive care unit. Nasopharyngeal aspirate samples of all patients in the neonatal intensive care unit were tested for 17 respiratory viruses using real-time reverse transcriptase polymerase chain reaction. Genotyping was performed using nucleotide sequencing. Results: From May to August 2013, two different outbreaks were detected in the neonatal intensive care unit. A total of 20 infants were infected with respiratory syncytial virus-A (ten and 14 with ON-1 and NA-2 genotypes, respectively). The mean age of the infants was 10 days, mean birth weight was 1,961g, and the mean gestational age was 33 weeks. Risk factors (heart disease, lung disease, and prematurity) were present in 80% and 85.7% of infants in the ON-1 and NA-2 groups, respectively. In total, 45.8% of infants were asymptomatic and 20.8% required mechanical ventilation. Coinfections were not detected during the outbreaks. Conclusions: Infants in a neonatal intensive care unit who develop abrupt respiratory symptoms should be tested for respiratory viruses, especially respiratory syncytial virus. Even in the absence of severe symptoms, respiratory syncytial virus detection can prevent nosocomial transmission through infection control measures. A better understanding of respiratory syncytial virus molecular epidemiology is essential for developing new vaccines and antiviral drugs against respiratory syncytial virus. (Copyright © 2018 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.)
Databáze:	MEDLINE
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