Improved dentin disinfection by combining different-geometry rotary nickel-titanium files in preparing root canals.

Autor:	Bedier MM; Department of Endodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt., Hashem AAR; Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt., Hassan YM; Department of Clinical Pathology, Cairo University, Cairo, Egypt.
Jazyk:	angličtina
Zdroj:	Restorative dentistry & endodontics [Restor Dent Endod] 2018 Nov 01; Vol. 43 (4), pp. e46. Date of Electronic Publication: 2018 Nov 01 (Print Publication: 2018).
DOI:	10.5395/rde.2018.43.e46
Abstrakt:	Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis ( E. faecalis ). Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis . The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of 50 µm inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons. Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction ( p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/XPF and XPS/C group, while the iRaCe/C group had the weakest effect. Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of 50 µm in the dentinal tubules. Competing Interests: Conflict of Interest: No potential conflict of interest relevant to this article was reported.
Databáze:	MEDLINE
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