Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation.

Autor: Castilho-Fernandes A; Department of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, Ribeirão Preto, University of São Paulo, 14040-901, Brazil., Lopes TG; Department of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, Ribeirão Preto, University of São Paulo, 14040-901, Brazil., Ferreira FU; Center for Cell Therapy and Regional Blood Center, National Institute of Science and Technology in Stem Cell and Cell Therapy, Medical School, University of São Paulo, Ribeirão Preto 14051-140, Brazil., Rezende N; Department of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, Ribeirão Preto, University of São Paulo, 14040-901, Brazil., Silva VF; Department of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, Ribeirão Preto, University of São Paulo, 14040-901, Brazil; Center for Cell Therapy and Regional Blood Center, National Institute of Science and Technology in Stem Cell and Cell Therapy, Medical School, University of São Paulo, Ribeirão Preto 14051-140, Brazil., Primo FL; Department of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, Ribeirão Preto, University of São Paulo, 14040-901, Brazil; Departament of Bioprocess and Biotechnology, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara SP 14800-903, Brazil., Fontes AM; Departament of de Genetics, Medical School, University of São Paulo, Ribeirão Preto 14051-140, Brazil., Ribeiro-Silva A; Department of Pathology, Medical School, University of Sao Paulo, Ribeirao Preto 14049-900, Brazil., Tedesco AC; Department of Chemistry, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, Ribeirão Preto, University of São Paulo, 14040-901, Brazil. Electronic address: atedesco@usp.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2019 Mar; Vol. 25, pp. 119-127. Date of Electronic Publication: 2018 Nov 17.
DOI: 10.1016/j.pdpdt.2018.11.013
Abstrakt: Background: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL).
Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP + ) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL.
Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 μmol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm 2 at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPARγ); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo.
Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photo-induced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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