Diversity in CO 2 -Concentrating Mechanisms among Chemolithoautotrophs from the Genera Hydrogenovibrio , Thiomicrorhabdus , and Thiomicrospira , Ubiquitous in Sulfidic Habitats Worldwide.

Autor:	Scott KM; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA kmscott@usf.edu., Leonard JM; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Boden R; School of Biological and Marine Sciences, University of Plymouth, Plymouth, United Kingdom.; Sustainable Earth Institute, University of Plymouth, Plymouth, United Kingdom., Chaput D; Proteomics and Mass Spectrometry Facility, University of South Florida, Tampa, Florida, USA., Dennison C; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Haller E; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Harmer TL; Biology Program, Stockton University, Galloway, New Jersey, USA., Anderson A; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Arnold T; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Budenstein S; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Brown R; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Brand J; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Byers J; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Calarco J; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Campbell T; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Carter E; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Chase M; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Cole M; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Dwyer D; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Grasham J; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Hanni C; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Hazle A; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Johnson C; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Johnson R; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Kirby B; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Lewis K; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Neumann B; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Nguyen T; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Nino Charari J; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Morakinyo O; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Olsson B; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Roundtree S; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Skjerve E; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Ubaldini A; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA., Whittaker R; Department of Integrative Biology, University of South Florida, Tampa, Florida, USA.
Jazyk:	angličtina
Zdroj:	Applied and environmental microbiology [Appl Environ Microbiol] 2019 Jan 23; Vol. 85 (3). Date of Electronic Publication: 2019 Jan 23 (Print Publication: 2019).
DOI:	10.1128/AEM.02096-18
Abstrakt:	Members of the genera Hydrogenovibrio , Thiomicrospira , and Thiomicrorhabdus fix carbon at hydrothermal vents, coastal sediments, hypersaline lakes, and other sulfidic habitats. The genome sequences of these ubiquitous and prolific chemolithoautotrophs suggest a surprising diversity of mechanisms for the uptake and fixation of dissolved inorganic carbon (DIC); these mechanisms are verified here. Carboxysomes are apparent in the transmission electron micrographs of most of these organisms but are lacking in Thiomicrorhabdus sp. strain Milos-T2 and Thiomicrorhabdus arctica , and the inability of Thiomicrorhabdus sp. strain Milos-T2 to grow under low-DIC conditions is consistent with the absence of carboxysome loci in its genome. For the remaining organisms, genes encoding potential DIC transporters from four evolutionarily distinct families (Tcr_0853 and Tcr_0854, Chr, SbtA, and SulP) are located downstream of carboxysome loci. Transporter genes collocated with carboxysome loci, as well as some homologs located elsewhere on the chromosomes, had elevated transcript levels under low-DIC conditions, as assayed by reverse transcription-quantitative PCR (qRT-PCR). DIC uptake was measureable via silicone oil centrifugation when a representative of each of the four types of transporter was expressed in Escherichia coli The expression of these genes in the carbonic anhydrase-deficient E. coli strain EDCM636 enabled it to grow under low-DIC conditions, a result consistent with DIC transport by these proteins. The results from this study expand the range of DIC transporters within the SbtA and SulP transporter families, verify DIC uptake by transporters encoded by Tcr_0853 and Tcr_0854 and their homologs, and introduce DIC as a potential substrate for transporters from the Chr family. IMPORTANCE Autotrophic organisms take up and fix DIC, introducing carbon into the biological portion of the global carbon cycle. The mechanisms for DIC uptake and fixation by autotrophic Bacteria and Archaea are likely to be diverse but have been well characterized only for " Cyanobacteria " Based on genome sequences, members of the genera Hydrogenovibrio , Thiomicrospira , and Thiomicrorhabdus have a variety of mechanisms for DIC uptake and fixation. We verified that most of these organisms are capable of growing under low-DIC conditions, when they upregulate carboxysome loci and transporter genes collocated with these loci on their chromosomes. When these genes, which fall into four evolutionarily independent families of transporters, are expressed in E. coli , DIC transport is detected. This expansion in known DIC transporters across four families, from organisms from a variety of environments, provides insight into the ecophysiology of autotrophs, as well as a toolkit for engineering microorganisms for carbon-neutral biochemistries of industrial importance. (Copyright © 2019 American Society for Microbiology.)
Databáze:	MEDLINE
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