| Autor: |
Pereira ASBF; Post-Graduation Program in Oral Science, Department of Dentistry, UFRN, Natal 59072-970, Brazil. aly_line@hotmail.com., Brito GAC; Post-Graduation Program in Pharmacology/Post-Graduation Program in Morphology, Department of Morphology, UFC, Fortaleza 60440-900, Brazil. gerlybrito@hotmail.com., Lima MLS; Post-Graduation Program in Oral Science, Department of Dentistry, UFRN, Natal 59072-970, Brazil. mlauradesouzalima@gmail.com., Silva Júnior AAD; Post-Graduation Program in Pharmaceutical Science/Post-Graduation Program in Health Science, Department of Pharmacology, UFRN, Natal 59072-970, Brazil. arnobiosilva@gmail.com., Silva EDS; Post-Graduation Program in Pharmaceutical Science, UFRN, Department of Pharmacology, Natal 59072-970, Brazil. emanuell_111@hotmail.com., de Rezende AA; Post-Graduation Program in Pharmaceutical Science, UFRN, Department of Pharmacology, Natal 59072-970, Brazil. adrirezende@yahoo.com., Bortolin RH; Post-Graduation Program in Pharmaceutical Science, UFRN, Department of Pharmacology, Natal 59072-970, Brazil. raulhbortolin@yahoo.com.br., Galvan M; Periodontics Section, School of Dentistry, University of California, UCLA, Los Angeles, CA 90095, USA. mgalvan@dentistry.ucla.edu., Pirih FQ; Periodontics Section, School of Dentistry, University of California, UCLA, Los Angeles, CA 90095, USA. fpirih@dentistry.ucla.edu., Araújo Júnior RF; Post-Graduation Program in Functional and Structural Biology/Post-Graduation Program Health Science, Department of Morphology, UFRN, Natal 59072-970, Brazil. araujojra@cb.ufrn.br., Medeiros CACX; Post-Graduation Program Biological Science/Post-Graduation Program in RENORBIO, Department of Biophysics and Pharmacology, UFRN, Natal 59072-970, Brazil. carolineaddisonfarma@yahoo.com.br., Guerra GCB; Post-Graduation Program Biological Science/Post-Graduation Program in Pharmaceutical Science, Department of Biophysics and Pharmacology, UFRN, Natal 59072-970, Brazil. gerlaneguerra@hotmail.com., Araújo AA; Post-Graduation Program Oral Science/Post-Graduation Program in Pharmaceutical Science, Department of Biophysics and Pharmacology, UFRN, Natal 59072-970, Brazil. aurigena@ufrnet.br.; Departamento de Biofisica e Farmacologia, Av. Senador Salgado Filho, S/N, Campus Universitário, Lagoa Nova, UFRN, Natal 59072-970, Brazil. aurigena@ufrnet.br. |
| Abstrakt: |
Evidence shows that metformin is an antidiabetic drug, which can exert favorable anti-inflammatory effects and decreased bone loss. The development of nanoparticles for metformin might be useful for increased therapeutic efficacy. The aim of this study was to evaluate the effect of metformin hydrochloride-loaded Poly (d,l-Lactide- co -glycolide) (PLGA)/(MET-loaded PLGA) on a ligature-induced periodontitis model in diabetic rats. MET-loaded PLGA were characterized by mean diameter, particle size, polydispensity index, and entrapment efficiency. Maxillae were scanned using Microcomputed Tomography (µCT) and histopathological and immunohistochemical analysis. IL-1β and TNF-α levels were analyzed by ELISA immunoassay. Quantitative RT-PCR was used ( AMPK , NF-κB p65 , HMGB1 , and TAK-1 ). The mean diameter of MET-loaded PLGA nanoparticles was in a range of 457.1 ± 48.9 nm ( p < 0.05) with a polydispersity index of 0.285 ( p < 0.05), Z potential of 8.16 ± 1.1 mV ( p < 0.01), and entrapment efficiency (EE) of 66.7 ± 3.73. Treatment with MET-loaded PLGA 10 mg/kg showed low inflammatory cells, weak staining by RANKL, cathepsin K, OPG, and osteocalcin, and levels of IL-1β and TNF-α ( p < 0.05), increased AMPK expression gene ( p < 0.05) and decreased NF-κB p65 , HMGB1 , and TAK-1 ( p < 0.05). It is concluded that MET-loaded PLGA decreased inflammation and bone loss in periodontitis in diabetic rats. |
