| Autor: |
Karginov AV; Bach Institute of Biochemistry, Research Center of Biotechnology RAS, Moscow, Russian Federation., Fokina AV; Bach Institute of Biochemistry, Research Center of Biotechnology RAS, Moscow, Russian Federation., Kang HA; Department of Life Science, Chung-Ang University, Seoul, Republic of Korea., Kalebina TS; Department of Molecular Biology, Biological Faculty, Lomonosov Moscow State University, Moscow, Russian Federation., Sabirzyanova TA; Department of Molecular Biology, Biological Faculty, Lomonosov Moscow State University, Moscow, Russian Federation., Ter-Avanesyan MD; Bach Institute of Biochemistry, Research Center of Biotechnology RAS, Moscow, Russian Federation., Agaphonov MO; Bach Institute of Biochemistry, Research Center of Biotechnology RAS, Moscow, Russian Federation. agaphonov@inbi.ras.ru. |
| Abstrakt: |
The closely related yeasts Ogataea polymorpha and O. parapolymorpha differ drastically from each other by sensitivity to the toxic phosphate analog vanadate. Search for genes underlying this difference revealed two genes, one designated as ABV1 (Alcian Blue staining, Vanadate resistance), which encodes a homologue of Saccharomyces cerevisiae Mnn4 responsible for attachment of mannosylphosphate to glycoside chains of secretory proteins, and the other designated as its S. cerevisiae homologue PHO87, encoding the plasma membrane low affinity phosphate sensor/transporter. The effect of Pho87 on vanadate resistance was bidirectional, since it decreased the resistance on phosphate-depleted medium, but was required for pronounced protection against vanadate by external phosphate. This highlights the dual function of this protein as a low affinity phosphate transporter and an external phosphate sensor. Involvement of Pho87 in phosphate sensing was confirmed by its effects on regulation of the promoter of the PHO84 gene, encoding a high affinity phosphate transporter. The effect of Abv1 was also complex, since it influenced Pho87 level and enhanced repression of the PHO84 promoter via a Pho87-independent pathway. Role of the identified genes in the difference in vanadate resistance between O. polymorpha and O. parapolymorpha is discussed. |
