TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells.

Autor: Yen J; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Fiorino M; NIBR Informatics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Liu Y; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Paula S; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Clarkson S; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Quinn L; Biotherapeutic and Analytical Tech, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Tschantz WR; Biotherapeutic and Analytical Tech, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Klock H; Biotherapeutics & Biotechnology, The Genomics Institute of the Novartis Research Foundation, La Jolla, California, USA., Guo N; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Russ C; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Yu VWC; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Mickanin C; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Stevenson SC; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Lee C; Global Discovery Chemistry, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA., Yang Y; Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA. yi.yang@novartis.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Nov 02; Vol. 8 (1), pp. 16304. Date of Electronic Publication: 2018 Nov 02.
DOI: 10.1038/s41598-018-34601-6
Abstrakt: CRISPR/Cas9 mediated gene editing of patient-derived hematopoietic stem and progenitor cells (HSPCs) ex vivo followed by autologous transplantation of the edited HSPCs back to the patient can provide a potential cure for monogenic blood disorders such as β-hemoglobinopathies. One challenge for this strategy is efficient delivery of the ribonucleoprotein (RNP) complex, consisting of purified Cas9 protein and guide RNA, into HSPCs. Because β-hemoglobinopathies are most prevalent in developing countries, it is desirable to have a reliable, efficient, easy-to-use and cost effective delivery method. With this goal in mind, we developed TRansmembrane Internalization Assisted by Membrane Filtration (TRIAMF), a new method to quickly and effectively deliver RNPs into HSPCs by passing a RNP and cell mixture through a filter membrane. We achieved robust gene editing in HSPCs using TRIAMF and demonstrated that the multilineage colony forming capacities and the competence for engraftment in immunocompromised mice of HSPCs were preserved post TRIAMF treatment. TRIAMF is a custom designed system using inexpensive components and has the capacity to process HSPCs at clinical scale.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje