Evolutionary origin of 2A-like sequences in Totiviridae genomes.

Autor: de Lima JGS; Applied Molecular Biology Lab - LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Teixeira DG; Laboratory of Metabolic Systems and Bioinformatics - LASIS, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Freitas TT; Applied Molecular Biology Lab - LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Postgraduate Program in Science, Technology and Innovation, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Lima JPMS; Laboratory of Metabolic Systems and Bioinformatics - LASIS, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Lanza DCF; Applied Molecular Biology Lab - LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Postgraduate Program in Science, Technology and Innovation, Federal University of Rio Grande do Norte, Natal, RN, Brazil. Electronic address: danielclanza@gmail.com.
Jazyk: angličtina
Zdroj: Virus research [Virus Res] 2019 Jan 02; Vol. 259, pp. 1-9. Date of Electronic Publication: 2018 Oct 16.
DOI: 10.1016/j.virusres.2018.10.011
Abstrakt: In recent years there has been a significant increase in the number of new species potentially belonging to the Totiviridae family. Most of these new viruses have not yet been covered by the Committee on Taxonomy of Viruses (ICTV) official classification. In this study, a phylogenetic analysis including new sequences of Totiviridae candidates revealed a clade including Giardiavirus and a great diversity of new totiviruses, which infect arthropods, protozoa and mollusc. This expanded Giardiavirus clade comprises two monophyletic groups, one of them including Giardia lamblia virus (GLV) grouped with viruses that infect arthropods and vertebrates (GLV-like group), and the other includes the previously proposed Artivirus group (IMNV-like group). A screening of the members of the GLV-like group in search of genomic elements already described in IMNV-like group revealed the existence of sites with a high propensity to become 2 A-like oligopeptides, mainly in a specific subgroup of arthropod viruses, suggesting that these viruses preserved ancestral characteristics. The existence of these "pseudo 2 A-sites" associated to phylogenetic reconstruction indicates that these sequences appear at a decisive stage for viral evolution. If they are changed to functional 2 A-like sequences, an irreversible route to increase the genome complexity will be initiated.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: