Isolation and Characterization of Pancreatic Canine Fetal Cells at the Final Stage of Gestation.

Autor: Aguiar BA; Department of Surgery Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Orechio D; Department of Surgery Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Fratini P; Department of Surgery Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Carreira ACO; Department of Surgery Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.; NUCEL (Cell and Molecular Therapy Center), Internal Medical Department, School of Medicine, University of São Paulo, São Paulo, Brazil.; Interunits Graduate Program in Biotechnology, Institute of Biosciences, University of São Paulo University of São Paulo, São Paulo, Brazil., Castelucci P; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Miglino MA; Department of Surgery Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Anatomical record (Hoboken, N.J. : 2007) [Anat Rec (Hoboken)] 2019 Aug; Vol. 302 (8), pp. 1409-1418. Date of Electronic Publication: 2018 Nov 25.
DOI: 10.1002/ar.23995
Abstrakt: The incidence of diabetes mellitus in dogs is increasing in recent years, mainly because of genetic and/or environmental factors, including endocrine disorders (like in humans); failure of suitable control of blood sugar levels, which triggers hyperglycemia; glycosuria and weight loss, which demands the development of innovative treatments to cure or treat this complex disease in dogs. The present study established for the first time a protocol to obtain and characterize cells derived from pancreas of canine fetuses. Those fetuses do not have a defined breed and were at the final stage of gestation. The protocol aims to provide morphological data to enable future applications of these cells for therapeutic approaches. In cell culture, pancreatic cells showed a fibroblast-like appearance with a mono-layered growth pattern and were not tumorigenic. They exhibited a positive expression for the pluripotent proliferation markers NANOG and PCNA and expressed PDX1, a transcription factor that is important for activation of the insulin gene promoter. In addition, Tyrosine Hydroxylase-positive (TH+) sympathetic nerve fibers were identified. Histologically, the pancreatic epithelium was developed, pancreatic glands in the fetuses were like those in the parenchyma of postconception dogs and pancreatic islets were unevenly distributed and organized in small clusters along the glands close to the vasculature. Staining with dithizone indicated the presence of insulin in the cells. A large number of beta cells were confirmed by immunofluorescence. In conclusion, the canine fetal pancreas cells could be an alternative and adequate source of cell lineages for stem cell therapies for diabetes treatment. Anat Rec, 302:1409-1418, 2019. © 2018 Wiley Periodicals, Inc.
(© 2018 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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