Melatonin promotes reduction in TNF levels and improves the lipid profile and insulin sensitivity in pinealectomized rats with periodontal disease.

Autor: Santos RMD; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Brazil; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Marani F; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Brazil; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Chiba FY; Preventive and Social Dentistry Post-Graduation Program School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Mattera MSLC; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Brazil; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Tsosura TVS; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Brazil; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Tessarin GWL; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil; Institute of Biosciences of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Pereira RF; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Brazil; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Belardi BE; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Pinheiro BCES; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil., Sumida DH; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Brazil; Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil. Electronic address: dorishs@foa.unesp.br.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2018 Nov 15; Vol. 213, pp. 32-39. Date of Electronic Publication: 2018 Oct 12.
DOI: 10.1016/j.lfs.2018.09.056
Abstrakt: Aim: This study aimed to investigate the effects of melatonin (ME) on insulin resistance (IR) and signaling (IS), proinflammatory cytokine levels, and lipid profiles in pinealectomyzed (PNX) rats with periodontal disease (PD).
Main Methods: One hundred and forty-four rats (age = 40 days) were distributed into 8 groups: 1) control (CN); 2) PD only; 3) PNX only; 4) PNX and PD (PNXPD); 5) CN treated with ME (CNM); 6) PD treated with ME (PDM); 7) PNX treated with ME(PNXM); 8) PNX and PD treated with ME(PNXPDM). The PNX groups were subjected to pinealectomy at 40 and at 60 days of age. The animals were then subjected to PD induction in the mandibular first molars. After PD induction, the ME replacement therapy (MERT-5 mg/kg body weight) was performed using water for 28 days. After this period, the plasma concentration of glucose, insulin, TNF, IL-6, triglycerides, total cholesterol, HDL-cholesterol, LDL-cholesterol, and VLDL-cholesterol and the HOMA-IR index were determined. Akt serine phosphorylation status in the white adipose tissue, gastrocnemius muscle, and rat liver were also evaluated.
Key Findings: PD, PNX, and PNXPD groups showed an increase in IR with elevated plasma levels of insulin and TNF compared to CN group. PNX and PNXPD groups presented alteration in lipid profile compared to CN group. MERT improved all of the analyzed parameters. No difference was observed in the IS among different groups.
Significance: The results suggest that MERT efficiently prevents IR, improves lipid profile, and increases plasma levels of insulin and TNF in PD and PNX rats.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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