Whole-Body 12 C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate.

Autor: Zanni G; Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. giulia.zanni85@gmail.com., Deutsch HM; Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. hdeu@sas.upenn.edu.; Biological Basis of Behavior Program, University of Pennsylvania, Philadelphia, PA 19104-6270, USA. hdeu@sas.upenn.edu., Rivera PD; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. phrivera@gmail.com., Shih HY; Department of Radiation Oncology, UT Southwestern Medical Center, Dallas, TX 75390, USA. Eric.Shih@UTSouthwestern.edu., LeBlanc JA; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. junie_leblanc@hotmail.com., Amaral WZ; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. wamaral@wisc.edu., Lucero MJ; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. Mjlucero1@gmail.com., Redfield RL; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. rachelredfield22@gmail.com., DeSalle MJ; Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. desallem7@gmail.com., Chen BPC; Department of Radiation Oncology, UT Southwestern Medical Center, Dallas, TX 75390, USA. Benjamin.Chen@UTSouthwestern.edu., Whoolery CW; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. cody.whoolery@gmail.com., Reynolds RP; Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. ryan.p.reynolds07@gmail.com.; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. ryan.p.reynolds07@gmail.com., Yun S; Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. YUNS@email.chop.edu.; Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. YUNS@email.chop.edu., Eisch AJ; Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. eischa@email.chop.edu.; Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75390, USA. eischa@email.chop.edu.; Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. eischa@email.chop.edu.; Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. eischa@email.chop.edu.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2018 Oct 09; Vol. 19 (10). Date of Electronic Publication: 2018 Oct 09.
DOI: 10.3390/ijms19103078
Abstrakt: High-charge and -energy (HZE) particles comprise space radiation and they pose a challenge to astronauts on deep space missions. While exposure to most HZE particles decreases neurogenesis in the hippocampus-a brain structure important in memory-prior work suggests that 12 C does not. However, much about 12 C's influence on neurogenesis remains unknown, including the time course of its impact on neurogenesis. To address this knowledge gap, male mice (9⁻11 weeks of age) were exposed to whole-body 12 C irradiation 100 cGy (IRR; 1000 MeV/n; 8 kEV/µm) or Sham treatment. To birthdate dividing cells, mice received BrdU i.p. 22 h post-irradiation and brains were harvested 2 h (Short-Term) or three months (Long-Term) later for stereological analysis indices of dentate gyrus neurogenesis. For the Short-Term time point, IRR mice had fewer Ki67, BrdU, and doublecortin (DCX) immunoreactive (+) cells versus Sham mice, indicating decreased proliferation (Ki67, BrdU) and immature neurons (DCX). For the Long-Term time point, IRR and Sham mice had similar Ki67+ and DCX+ cell numbers, suggesting restoration of proliferation and immature neurons 3 months post- 12 C irradiation. IRR mice had fewer surviving BrdU+ cells versus Sham mice, suggesting decreased cell survival, but there was no difference in BrdU+ cell survival rate when compared within treatment and across time point. These data underscore the ability of neurogenesis in the mouse brain to recover from the detrimental effect of 12 C exposure.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje