Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model.

Autor: Colombatti Olivieri MA; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Biotecnología, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: colombatti.alejandra@inta.gob.ar., Moyano RD; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Biotecnología, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina., Traveria GE; Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE) de la Facultad de Ciencias Veterinarias - Universidad de La Plata, Argentina., Alvarado Pinedo MF; Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE) de la Facultad de Ciencias Veterinarias - Universidad de La Plata, Argentina., Mon ML; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Biotecnología, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina., Gravisaco MJ; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Biotecnología, Argentina., Delgado FO; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Patobiología, Argentina., Santangelo MP; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Biotecnología, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina., Romano MI; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Biotecnología, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2018 Dec; Vol. 121, pp. 4-11. Date of Electronic Publication: 2018 Oct 02.
DOI: 10.1016/j.rvsc.2018.09.009
Abstrakt: Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and immune response of Map strains that could be used as a vaccine. This study evaluates the degree of virulence, immune response, and protection of Argentinian strains of Map with different genotype in a murine model. Four local isolates (Cattle type) with different genotypes (analyzed by MIRU-VNTR and SSRs) were selected and evaluated in a virulence assay in BALB/c mice. This assay allowed us to differentiate virulent and low-virulence Map strains. The less virulent strains (1543/481 and A162) failed to induce a significant production of the proinflammatory cytokine IFNg, whereas the virulent strain 6611 established infection along with a proinflammatory immune response. On the other hand, the virulent strain 1347/498 was efficient in establishing a persistent infection, but failed to promote an important Th1 response compared with 6611 at the evaluated time. We selected the low-virulence strain 1543/498 as a live vaccine and the virulent strain 6611 as a live and inactivated vaccine in a protection assay in mice. Strain 1543/481 failed to protect the animals from challenge, whereas strain 6611, in its live and inactivated form, significantly reduced the CFUs count in the infected mice, although they had different immunological response profiles. The inactivated virulent strain 6611 is a potential vaccine candidate against paratuberculosis to be tested in cattle.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: