| Autor: |
Kwon DH; Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Republic of Korea., Park JB; Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Republic of Korea., Hong E; Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, USA., Ha SJ; Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Republic of Korea. sjha@kangwon.ac.kr. |
| Jazyk: |
angličtina |
| Zdroj: |
Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2019 Jan; Vol. 42 (1), pp. 63-70. Date of Electronic Publication: 2018 Sep 22. |
| DOI: |
10.1007/s00449-018-2014-0 |
| Abstrakt: |
Directed evolutionary approach and random mutagenesis were performed on thermotolerant yeast Kluyveromyces marxianus KCTC17694 for isolating a yeast strain producing ethanol from xylose efficiently. The isolated mutant strain, K. marxianus 17694-DH1, showed 290% and 131% improvement in ethanol concentration and ethanol production yield from xylose, respectively, as compared with the parental strain. Sequencing of the KmXYL1 gene of K. marxianus 17694-DH1 revealed substitutions of arginine and tryptophan with lysine and leucine at positions 25 and 202, respectively, as compared to the parental strain. In addition, sequencing of the KmXYL2 gene uncovered a substitution of glutamate with leucine at position 232. When enzymatic assays of xylose reductase (XR) and xylitol dehydrogenase (XDH) from the parental strain and K. marxianus 17694-DH1 were performed, XR activities were not significantly different whereas XDH activities were significantly improved in the mutant strain up to 50 °C of reaction temperatures. RNA-Seq based transcriptome analysis showed that alcohol dehydrogenases and glucose transporters were up-regulated while TCA cycle involved enzymes were down-regulated in K. marxianus 17694-DH1. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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