Schistosoma mansoni schistosomula antigens induce Th1/Pro-inflammatory cytokine responses.

Autor: Egesa M; Department of Medical Microbiology, School of Biomedical Sciences, Makerere University College of Health Sciences, Kampala, Uganda.; Medical Research Council/Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine Uganda Research Unit, Entebbe, Uganda., Lubyayi L; Medical Research Council/Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine Uganda Research Unit, Entebbe, Uganda., Tukahebwa EM; Vector Control Division, Ministry of Health, Kampala, Uganda., Bagaya BS; Department of Immunology and Molecular Biology, School of Biomedical Sciences, Makerere University College of Health Sciences, Kampala, Uganda., Chalmers IW; Institute of Biological, Environmental & Rural Sciences, Aberystwyth University, Aberystwyth, UK., Wilson S; Department of Pathology, University of Cambridge, Cambridge, UK., Hokke CH; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Hoffmann KF; Institute of Biological, Environmental & Rural Sciences, Aberystwyth University, Aberystwyth, UK., Dunne DW; Department of Pathology, University of Cambridge, Cambridge, UK., Yazdanbakhsh M; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Labuda LA; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Cose S; Medical Research Council/Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine Uganda Research Unit, Entebbe, Uganda.; Department of Clinical Research, London School of Hygiene & Tropical Medicine, London, UK.
Jazyk: angličtina
Zdroj: Parasite immunology [Parasite Immunol] 2018 Dec; Vol. 40 (12), pp. e12592. Date of Electronic Publication: 2018 Oct 21.
DOI: 10.1111/pim.12592
Abstrakt: Larvae of Schistosoma (schistosomula) are highly susceptible to host immune responses and are attractive prophylactic vaccine targets, although cellular immune responses against schistosomula antigens in endemic human populations are not well characterized. We collected blood and stool from 54 Schistosoma mansoni-infected Ugandans, isolated peripheral blood mononuclear cells and stimulated them for 24 hours with schistosome adult worm and soluble egg antigens (AWA and SEA), along with schistosomula recombinant proteins rSmKK7, Lymphocyte Antigen 6 isoforms (rSmLy6A and rSmLy6B), tetraspanin isoforms (rSmTSP6 and rSmTSP7). Cytokines, chemokines and growth factors were measured in the culture supernatants using a multiplex luminex assay, and infection intensity was determined before and at 1 year after praziquantel (PZQ) treatment using the Kato-Katz method. Cellular responses were grouped and the relationship between groups of correlated cellular responses and infection intensity before and after PZQ treatment was investigated. AWA and SEA induced mainly Th2 responses. In contrast, rSmLy6B, rSmTSP6 and rSmTSP7 induced Th1/pro-inflammatory responses. While recombinant antigens rSmKK7 and rSmLy6A did not induce a Th1/pro-inflammatory response, they had an association with pre-treatment infection intensity after adjusting for age and sex. Testing more schistosomula antigens using this approach could provide immune-epidemiology identifiers necessary for prioritizing next generation schistosomiasis vaccine candidates.
(© 2018 The Authors. Parasite Immunology Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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