Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop.

Autor: McGinn M; School of Biological Sciences, Illinois State University, Normal, IL, USA., Phippen WB; School of Agriculture, Western Illinois University, Macomb, IL, USA., Chopra R; Department of Plant Biology, University of Minnesota, Saint Paul, MN, USA., Bansal S; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, USA., Jarvis BA; School of Biological Sciences, Illinois State University, Normal, IL, USA., Phippen ME; School of Agriculture, Western Illinois University, Macomb, IL, USA., Dorn KM; Department of Plant Biology, University of Minnesota, Saint Paul, MN, USA., Esfahanian M; School of Biological Sciences, Illinois State University, Normal, IL, USA., Nazarenus TJ; Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA., Cahoon EB; Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA., Durrett TP; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, USA., Marks MD; Department of Plant Biology, University of Minnesota, Saint Paul, MN, USA., Sedbrook JC; School of Biological Sciences, Illinois State University, Normal, IL, USA.
Jazyk: angličtina
Zdroj: Plant biotechnology journal [Plant Biotechnol J] 2019 Apr; Vol. 17 (4), pp. 776-788. Date of Electronic Publication: 2018 Oct 25.
DOI: 10.1111/pbi.13014
Abstrakt: Thlapsi arvense L. (pennycress) is being developed as a profitable oilseed cover crop for the winter fallow period throughout the temperate regions of the world, controlling soil erosion and nutrients run-off on otherwise barren farmland. We demonstrate that pennycress can serve as a user-friendly model system akin to Arabidopsis that is well-suited for both laboratory and field experimentation. We sequenced the diploid genome of the spring-type Spring 32-10 inbred line (1C DNA content of 539 Mb; 2n = 14), identifying variation that may explain phenotypic differences with winter-type pennycress, as well as predominantly a one-to-one correspondence with Arabidopsis genes, which makes translational research straightforward. We developed an Agrobacterium-mediated floral dip transformation method (0.5% transformation efficiency) and introduced CRISPR-Cas9 constructs to produce indel mutations in the putative FATTY ACID ELONGATION1 (FAE1) gene, thereby abolishing erucic acid production and creating an edible seed oil comparable to that of canola. We also stably transformed pennycress with the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene, producing low-viscosity acetyl-triacylglycerol-containing seed oil suitable as a diesel-engine drop-in fuel. Adoption of pennycress as a model system will accelerate oilseed-crop translational research and facilitate pennycress' rapid domestication to meet the growing sustainable food and fuel demands.
(© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje