| Autor: |
de Alcântara Sica de Toledo L; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Avenida Colombo, n. 5790, K80, S22, Maringa, Parana, 87020-900, Brazil., Rosseto HC; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Avenida Colombo, n. 5790, K80, S22, Maringa, Parana, 87020-900, Brazil., Dos Santos RS; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Avenida Colombo, n. 5790, K80, S22, Maringa, Parana, 87020-900, Brazil., Spizzo F; Department of Physics and Earth Sciences, University of Ferrara, Ferrara, Italy., Del Bianco L; Department of Physics and Earth Sciences, University of Ferrara, Ferrara, Italy., Montanha MC; Postgraduate Program in Biosciences and Physiopathology, State University of Maringa, Maringa, Parana, Brazil., Esposito E; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy., Kimura E; Postgraduate Program in Biosciences and Physiopathology, State University of Maringa, Maringa, Parana, Brazil., Bonfim-Mendonça PS; Laboratory of Medical Mycology, Department of Clinical Analysis and Biomedicine, State University of Maringa, Maringa, Parana, Brazil., Svidzinski TIE; Postgraduate Program in Biosciences and Physiopathology, State University of Maringa, Maringa, Parana, Brazil.; Laboratory of Medical Mycology, Department of Clinical Analysis and Biomedicine, State University of Maringa, Maringa, Parana, Brazil., Cortesi R; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy., Bruschi ML; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Avenida Colombo, n. 5790, K80, S22, Maringa, Parana, 87020-900, Brazil. mlbruschi@uem.br. |
| Abstrakt: |
Intra-periodontal pocket drug delivery systems, such as liquid crystalline systems, are widely utilized improving the drug release control and the therapy. Propolis is used in the treatment of periodontal diseases, reducing the inflammatory and infectious conditions. Iron oxide magnetic nanoparticles (MNPs) can improve the treatment when an alternating external magnetic field (AEMF) is applied, increasing the local temperature. The aim of this study was to develop a liquid crystalline system containing MNPs for intra-periodontal pocket propolis release. MNPs were prepared using iron salts and the morphological, size, thermal, x-ray diffraction, magnetometry, and Mössbauer spectroscopy analyses were performed. Cytotoxicity studies using Artemia salina and fibroblasts were also accomplished. The systems were prepared using polyoxyethylene (10) oleyl ether, isopropyl myristate, purified water, and characterized by polarized optical microscopy, rheometry, and in vitro drug release profile using a periodontal pocket simulator apparatus. The antifungal activity of the systems was investigated against Candida spp. using an AEMF. MNPs displayed nanometric size, were monodisperse, and they displayed very low cytotoxicity. Microscopically homogeneous formulations were obtained displaying important physicochemical and biological properties. The system displayed prolonged release of propolis and important in vitro fungicide activity, which was increased when the AEMF was applied, indicating a potentially alternative therapy for the treatment of the periodontal disease. |
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