Simultaneous transcriptome and proteome analysis of EA.hy926 cells under stress conditions induced by nanomaterials.

Autor:	Komorowski P; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland.; Department of Biophysics, Institute of Materials Science, Lodz University of Technology, Lodz, Poland.; BioTechMed Advanced Technology Centre, Lodz, Poland., Siatkowska M; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland., Wasiak T; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland., Działoszyńska K; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland.; Department of Biophysics, Institute of Materials Science, Lodz University of Technology, Lodz, Poland., Kotarba S; Biosensor and Organic Electronics Laboratory, Bionanopark Ltd., Lodz, Poland., Kądzioła K; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland., Bartoszek N; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland., Sokołowska P; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland., Elgalal M; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland.; Department of Diagnostic Imaging, Radiation and Isotope Therapy, Medical University of Lodz, Lodz, Poland., Sobol-Pacyniak A; Department of Internal Medicine and Diabetology, Medical University of Lodz, Lodz, Poland.; Norbert Barlicki Memorial Teaching Hospital No. 1, Lodz, Poland., Makowski K; Department of Biophysics, Institute of Materials Science, Lodz University of Technology, Lodz, Poland.; Industrial Biotechnology Laboratory, Bionanopark Ltd, Lodz, Poland., Walkowiak B; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Lodz, Poland.; Department of Biophysics, Institute of Materials Science, Lodz University of Technology, Lodz, Poland.; BioTechMed Advanced Technology Centre, Lodz, Poland.; Industrial Biotechnology Laboratory, Bionanopark Ltd, Lodz, Poland.
Jazyk:	angličtina
Zdroj:	Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2019 May; Vol. 107 (4), pp. 1024-1034. Date of Electronic Publication: 2018 Sep 10.
DOI:	10.1002/jbm.b.34195
Abstrakt:	Today, the extensive and constantly growing number of applications in the field of nanotechnology poses a lot of questions about the potential toxicity of nanomaterials (NMs) toward cells of different origins. In our work we employed the tools of molecular biology to evaluate changes that occur in human endothelial cells at the transcriptomic and proteomic level, following 24 h of exposure to three different classes of NMs. Using microarray technology, we demonstrated that 24 h of exposure to silver nanoparticles (SNPs), multiwalled carbon nanotubes (MWCNTs) and polyamidoamine dendrimers (PAMAMs) leads to changes in 299, 1271, and 431 genes, respectively, influencing specific molecular pathways. The 2D-DIGE and mass spectrometry analysis revealed that differentially expressed proteins were involved in numerous cellular processes, for example, cytoskeletal reorganization, cell growth and proliferation, or response to stress. Both, transcriptome and proteome alterations indicate reorganization of mechanism regulating cell functioning. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1024-1034, 2019. (© 2018 Wiley Periodicals, Inc.)
Databáze:	MEDLINE
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