Genome-wide association study provides insights into genes related with horn development in Nelore beef cattle.

Autor: Stafuzza NB; Departamento de Ciencias Exatas, Faculdade de Ciencias Agrarias e Veterinarias (FCAV), Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Jaboticabal, Sao Paulo, Brazil.; Department of Animal and Dairy Science, University of Georgia, Athens, Georgia, United States of America., Silva RMO; Department of Animal and Dairy Science, University of Georgia, Athens, Georgia, United States of America.; National Center for Cool and Cold Water Aquaculture (NCCCWA), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), Leetown, West Virginia, United States of America., Peripolli E; Departamento de Zootecnia, Faculdade de Ciencias Agrarias e Veterinarias (FCAV), Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Jaboticabal, Sao Paulo, Brazil., Bezerra LAF; Departamento de Genetica, Faculdade de Medicina de Ribeirao Preto (FMRP), Universidade de Sao Paulo (USP), Ribeirao Preto, Sao Paulo, Brazil., Lôbo RB; Associaçao Nacional dos Criadores e Pesquisadores (ANCP), Ribeirao Preto, Sao Paulo, Brazil., Magnabosco CU; Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Planaltina, Distrito Federal, Brazil., Di Croce FA; Zoetis, Kalamazoo, Michigan, United States of America., Osterstock JB; Zoetis, Kalamazoo, Michigan, United States of America., Munari DP; Departamento de Ciencias Exatas, Faculdade de Ciencias Agrarias e Veterinarias (FCAV), Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Jaboticabal, Sao Paulo, Brazil., Lourenco DAL; Department of Animal and Dairy Science, University of Georgia, Athens, Georgia, United States of America., Baldi F; Departamento de Zootecnia, Faculdade de Ciencias Agrarias e Veterinarias (FCAV), Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Jaboticabal, Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Aug 30; Vol. 13 (8), pp. e0202978. Date of Electronic Publication: 2018 Aug 30 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0202978
Abstrakt: The causal mutation for polledness in Nelore (Bos taurus indicus) breed seems to have appeared first in Brazil in 1957. The expression of the polled trait is known to be ruled by a few groups of alleles in taurine breeds; however, the genetic basis of this trait in indicine cattle is still unclear. The aim of this study was to identify genomic regions associated with the hornless trait in a commercial Nelore population. A total of 107,294 animals had phenotypes recorded and 2,238 were genotyped/imputed for 777k SNP. The weighted single-step approach for genome-wide association study (WssGWAS) was used to estimate the SNP effects and variances accounted for by 1 Mb sliding SNP windows. A centromeric region of chromosome 1 with 3.11 Mb size (BTA1: 878,631-3,987,104 bp) was found to be associated with hornless in the studied population. A total of 28 protein-coding genes are mapped in this region, including the taurine Polled locus and the IFNAR1, IFNAR2, IFNGR2, KRTAP11-1, MIS18A, OLIG1, OLIG2, and SOD1 genes, which expression can be related to the horn formation as described in literature. The functional enrichment analysis by DAVID tool revealed cytokine-cytokine receptor interaction, JAK-STAT signaling, natural killer cell mediated cytotoxicity, and osteoclast differentiation pathways as significant (P < 0.05). In addition, a runs of homozygosity (ROH) analysis identified a ROH island in polled animals with 2.47 Mb inside the region identified by WssGWAS. Polledness in Nelore cattle is associated with one region in the genome with 3.1 Mb size in chromosome 1. Several genes are harbored in this region, and they may act together in the determination of the polled/horned phenotype. Fine mapping the locus responsible for polled trait in Nelore breed and the identification of the molecular mechanisms regulating the horn growth deserve further investigation.
Competing Interests: Zoetis provided support in the form of salaries for authors FADC and JBO. The commercial affiliation of authors FADC and JBO does not alter our adherence to PLOS ONE policies on sharing data and materials. The authors declare that they have no competing interests.
Databáze: MEDLINE
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