Lactoperoxidase immobilization on silver nanoparticles enhances its antimicrobial activity.

Autor:	Sheikh IA; King Fahd Medical Research Center, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Yasir M; King Fahd Medical Research Center, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Khan I; King Fahd Medical Research Center, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Khan SB; Department of Chemistry,Faculty of Science,Center of Excellence for Advanced Materials Research, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Azum N; Department of Chemistry,Faculty of Science,Center of Excellence for Advanced Materials Research, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Jiffri EH; Faculty of Applied Medical Sciences,King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Kamal MA; King Fahd Medical Research Center, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Ashraf GM; King Fahd Medical Research Center, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia., Beg MA; King Fahd Medical Research Center, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia.
Jazyk:	angličtina
Zdroj:	The Journal of dairy research [J Dairy Res] 2018 Nov; Vol. 85 (4), pp. 460-464. Date of Electronic Publication: 2018 Aug 23.
DOI:	10.1017/S0022029918000730
Abstrakt:	Lactoperoxidase (LPO) is an antimicrobial protein present in milk that plays an important role in natural defence mechanisms during neonatal and adult life. The antimicrobial activity of LPO has been commercially adapted for increasing the shelf life of dairy products. Immobilization of LPO on silver nanoparticles (AgNPs) is a promising way to enhance the antimicrobial activity of LPO. In the current study, LPO was immobilized on AgNPs to form LPO/AgNP conjugate. The immobilized LPO/AgNP conjugate was characterized by various biophysical techniques. The enhanced antibacterial activity of the conjugate was tested against E. coli in culture at 2 h intervals for 10 h. The results showed successful synthesis of spherical AgNPs. LPO was immobilized on AgNPs with agglomerate sizes averaging approximately 50 nm. The immobilized conjugate exhibited stronger antibacterial activity against E. coli in comparison to free LPO. This study may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO system in dairy and other industries.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu