Immunological profile of teeth with inflammatory periapical disease from chronic liver disease patients.

Autor: Braga Diniz JM; Department of Operative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil., Espaladori MC; Department of Operative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil., Souza E Silva ME; Department of Operative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil., Brito LCN; Faculty of Dentistry, University of Itaúna, Itaúna, Brazil., Vieira LQ; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Ribeiro Sobrinho AP; Department of Operative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Jazyk: angličtina
Zdroj: International endodontic journal [Int Endod J] 2019 Feb; Vol. 52 (2), pp. 149-157. Date of Electronic Publication: 2018 Aug 29.
DOI: 10.1111/iej.12992
Abstrakt: Aim: To evaluate the mRNA expression levels of the cytokines interferon-γ, tumour necrosis factor-α, interleukin (IL)-1β, IL-10, IL-6, VEGF, and AGT and the chemokine CCL2/MCP-1 in periapical interstitial fluid associated with root canal infections before and after the reduction of the bacterial load using a cleaning procedure.
Methodology: The case group included 11 patients with chronic liver disease, and the control group included 11 healthy patients. Clinical samples were taken from teeth with pulp necrosis. After cleaning and drying the canal, three paper points were introduced into the root canal and passed through the root apex (2 mm) into the periapical tissues for 1 min. The samples were collected immediately after root canal cleaning and 7 days later to characterize those gene expression levels using real-time PCR. The data were subjected to the Shapiro-Wilk and the Wilcoxon tests.
Results: In the control group, significantly increased expression of the pro-inflammatory cytokines IFN-γ and TNF-α was observed in teeth with restrained bacterial loads (day 7) (P < 0.05). Similarly, increased TNF-α expression was found on day 7 in the liver group (P < 0.05). No differences were observed in the expression levels of the IL-1β, IL-10 and, IL-6, MCP-1/CCL-2 and VEGF between the first collection (day 0) and second collection (day 7), over time in either group.
Conclusion: Chronic liver disease patients exhibited sufficient immunologic ability showing relatively similar expression levels of cytokines, chemokines and angiogenic factors in periapical samples compared with the responses from no-chronic liver disease patients. The outcomes of this study suggest that liver impairment did not compromise the periapical immune response.
(© 2018 International Endodontic Journal. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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