Human pharyngeal microbiota in age-related macular degeneration.

Autor: Ho EXP; Genome Institute of Singapore,Singapore, Singapore., Cheung CMG; Singapore Eye Research Institute, Singapore National Eye Center, Duke-NUS Medical School, National University of Singapore, Singapore, Singapore., Sim S; Genome Institute of Singapore,Singapore, Singapore., Chu CW; Genome Institute of Singapore,Singapore, Singapore., Wilm A; Genome Institute of Singapore,Singapore, Singapore., Lin CB; Genome Institute of Singapore,Singapore, Singapore., Mathur R; Singapore Eye Research Institute, Singapore National Eye Center, Duke-NUS Medical School, National University of Singapore, Singapore, Singapore., Wong D; Singapore Eye Research Institute, Singapore National Eye Center, Duke-NUS Medical School, National University of Singapore, Singapore, Singapore., Chan CM; Singapore Eye Research Institute, Singapore National Eye Center, Duke-NUS Medical School, National University of Singapore, Singapore, Singapore., Bhagarva M; Department of Ophthalmology, National University of Singapore and National University Health System, Singapore, Singapore., Laude A; National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore, Singapore., Lim TH; National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore, Singapore., Wong TY; Singapore Eye Research Institute, Singapore National Eye Center, Duke-NUS Medical School, National University of Singapore, Singapore, Singapore., Cheng CY; Singapore Eye Research Institute, Singapore National Eye Center, Duke-NUS Medical School, National University of Singapore, Singapore, Singapore., Davila S; Genome Institute of Singapore,Singapore, Singapore., Hibberd M; Genome Institute of Singapore,Singapore, Singapore.; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Aug 08; Vol. 13 (8), pp. e0201768. Date of Electronic Publication: 2018 Aug 08 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0201768
Abstrakt: Background: While the aetiology of age-related macular degeneration (AMD)-a major blinding disease-remains unknown, the disease is strongly associated with variants in the complement factor H (CFH) gene. CFH variants also confer susceptibility to invasive infection with several bacterial colonizers of the nasopharyngeal mucosa. This shared susceptibility locus implicates complement deregulation as a common disease mechanism, and suggests the possibility that microbial interactions with host complement may trigger AMD. In this study, we address this possibility by testing the hypothesis that AMD is associated with specific microbial colonization of the human nasopharynx.
Results: High-throughput Illumina sequencing of the V3-V6 region of the microbial 16S ribosomal RNA gene was used to comprehensively and accurately describe the human pharyngeal microbiome, at genus level, in 245 AMD patients and 386 controls. Based on mean and differential microbial abundance analyses, we determined an overview of the pharyngeal microbiota, as well as candidate genera (Prevotella and Gemella) suggesting an association towards AMD health and disease conditions.
Conclusions: Utilizing an extensive study population from Singapore, our results provided an accurate description of the pharyngeal microbiota profiles in AMD health and disease conditions. Through identification of candidate genera that are different between conditions, we provide preliminary evidence for the existence of microbial triggers for AMD. Ethical approval for this study was obtained through the Singapore Health Clinical Institutional Review Board, reference numbers R799/63/2010 and 2010/585/A.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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