| Autor: |
Rodrigues Pires Júnior O; Toxinology Laboratory, Depto. Physiological Sciences, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. osmindo@unb.br., de Oliveira NB; Toxinology Laboratory, Depto. Physiological Sciences, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. natiela@gmail.com., Bosque RJ; Depto. Genetics and Morphology, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. rjbosque@go.olemiss.edu., Nice Ferreira MF; Depto. Genetics and Morphology, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. mfnf@unb.br., Morais Aurélio da Silva V; Toxinology Laboratory, Depto. Physiological Sciences, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. veronicasilva@gmail.com., Martins Magalhães AC; Toxinology Laboratory, Depto. Physiological Sciences, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. bioana.11@gmail.com., Correia de Santana CJ; Toxinology Laboratory, Depto. Physiological Sciences, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. carlosjcsantana@gmail.com., de Souza Castro M; Toxinology Laboratory, Depto. Physiological Sciences, Institute of Biology, University of Brasilia, Brasilia 70910-900, Brazil. mscastro69@gmail.com. |
| Abstrakt: |
This study evaluated the effects of [d-Leu¹]Microcystin-LR variant by the exposure of Lithobates catesbeianus tadpole to unialgal culture Microcystis aeruginosa NPLJ-4 strain. The Tadpole was placed in aquariums and exposed to Microcystis aeruginosa culture or disrupted cells. For 16 days, 5 individuals were removed every 2 days, and tissue samples of liver, skeletal muscle, and intestinal tract were collected for histopathology and bioaccumulation analyses. After exposure, those surviving tadpoles were placed in clean water for 15 days to evaluate their recovery. A control without algae and toxins was maintained in the same conditions and exhibited normal histology and no tissue damage. In exposed tadpoles, samples were characterized by serious damages that similarly affected the different organs, such as loss of adhesion between cells, nucleus fragmentation, necrosis, and hemorrhage. Samples showed signs of recovery but severe damages were still observed. Neither HPLC-PDA nor mass spectrometry analysis showed any evidence of free Microcystins bioaccumulation. |
