Evidence for Host-Bacterial Co-evolution via Genome Sequence Analysis of 480 Thai Mycobacterium tuberculosis Lineage 1 Isolates.

Autor: Palittapongarnpim P; Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand. Prasit.pal@mahidol.ac.th.; National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand. Prasit.pal@mahidol.ac.th., Ajawatanawong P; Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand., Viratyosin W; National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand., Smittipat N; National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand., Disratthakit A; Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi, Thailand., Mahasirimongkol S; Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi, Thailand., Yanai H; TB-HIV Research Foundation, Chiangrai, Thailand.; Fukujuji Hospital, Japan Anti-tuberculosis Association (JATA), Kiyose, Japan., Yamada N; Research Institute of Tuberculosis, JATA, Kiyose, Japan., Nedsuwan S; Chiangrai Prachanukroh Hospital, Ministry of Public Health, Chiangrai, Thailand., Imasanguan W; Chiangrai Prachanukroh Hospital, Ministry of Public Health, Chiangrai, Thailand., Kantipong P; Chiangrai Prachanukroh Hospital, Ministry of Public Health, Chiangrai, Thailand., Chaiyasirinroje B; TB-HIV Research Foundation, Chiangrai, Thailand., Wongyai J; TB-HIV Research Foundation, Chiangrai, Thailand., Toyo-Oka L; Department of Human Genetics, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan., Phelan J; London School of Hygiene and Tropical Medicine, London, UK., Parkhill J; Welcome Trust Sanger Institute, Hinxton, Cambridge, UK., Clark TG; London School of Hygiene and Tropical Medicine, London, UK., Hibberd ML; London School of Hygiene and Tropical Medicine, London, UK., Ruengchai W; Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand., Palittapongarnpim P; Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand., Juthayothin T; National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand., Tongsima S; National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand., Tokunaga K; Department of Human Genetics, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Aug 02; Vol. 8 (1), pp. 11597. Date of Electronic Publication: 2018 Aug 02.
DOI: 10.1038/s41598-018-29986-3
Abstrakt: Tuberculosis presents a global health challenge. Mycobacterium tuberculosis is divided into several lineages, each with a different geographical distribution. M. tuberculosis lineage 1 (L1) is common in the high-burden areas in East Africa and Southeast Asia. Although the founder effect contributes significantly to the phylogeographic profile, co-evolution between the host and M. tuberculosis may also play a role. Here, we reported the genomic analysis of 480 L1 isolates from patients in northern Thailand. The studied bacterial population was genetically diverse, allowing the identification of a total of 18 sublineages distributed into three major clades. The majority of isolates belonged to L1.1 followed by L1.2.1 and L1.2.2. Comparison of the single nucleotide variant (SNV) phylogenetic tree and the clades defined by spoligotyping revealed some monophyletic clades representing EAI2_MNL, EAI2_NTM and EAI6_BGD1 spoligotypes. Our work demonstrates that ambiguity in spoligotype assignment could be partially resolved if the entire DR region is investigated. Using the information to map L1 diversity across Southeast Asia highlighted differences in the dominant strain-types in each individual country, despite extensive interactions between populations over time. This finding supported the hypothesis that there is co-evolution between the bacteria and the host, and have implications for tuberculosis disease control.
Databáze: MEDLINE
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