Spectroscopic Characterization of Natural Melanin from a Streptomyces cyaneofuscatus Strain and Comparison with Melanin Enzymatically Synthesized by Tyrosinase and Laccase.

Autor: Al Khatib M; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy. maher.alkhatib@student.unisi.it.; CSGI (Consorzio per lo Sviluppo dei Sistemi a Grande Interfase), 50019 Florence, Italy. maher.alkhatib@student.unisi.it., Harir M; Biology of Microorganisms and Biotechnology Laboratory, University of Oran 1 Ahmed ben Bella, BP1524, Oran El Mnaouer, 31000 Oran, Algeria. mohamedharir57@yahoo.fr.; Algeria and Faculty of Sciences, Natural and Life Sciences Department, Mohamed Boudiaf University, M'sila, Algeria. mohamedharir57@yahoo.fr., Costa J; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy. jessica.costa@student.unisi.it., Baratto MC; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy. mariacamilla.baratto@unisi.it.; CSGI (Consorzio per lo Sviluppo dei Sistemi a Grande Interfase), 50019 Florence, Italy. mariacamilla.baratto@unisi.it., Schiavo I; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy. schiavo6@student.unisi.it., Trabalzini L; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy. lorenza.trabalzini@unisi.it., Pollini S; Department of Experimental and Clinical Medicine, University of Florence and Clinical Microbiology and Virology Unit, Careggi University Hospital, 50134 Florence, Italy. simona.pollini@unifi.it., Rossolini GM; Department of Experimental and Clinical Medicine, University of Florence and Clinical Microbiology and Virology Unit, Careggi University Hospital, 50134 Florence, Italy. gianmaria.rossolini@unifi.it., Basosi R; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy. riccardo.basosi@unisi.it.; CSGI (Consorzio per lo Sviluppo dei Sistemi a Grande Interfase), 50019 Florence, Italy. riccardo.basosi@unisi.it., Pogni R; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy. rebecca.pogni@unisi.it.; CSGI (Consorzio per lo Sviluppo dei Sistemi a Grande Interfase), 50019 Florence, Italy. rebecca.pogni@unisi.it.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2018 Aug 01; Vol. 23 (8). Date of Electronic Publication: 2018 Aug 01.
DOI: 10.3390/molecules23081916
Abstrakt: An actinobacteria strain was isolated from Algerian Sahara soil and assigned to Streptomyces cyaneofuscatus Pridham et al. 1958 species. This strain was selected for its ability to produce melanin exopigments in liquid and solid media. Melanin synthesis was associated with tyrosinase activity and the enzyme from this strain was isolated and biochemically characterized. Synthetic melanin was then enzymatically produced using the S. cyaneofuscatus Pridham et al. 1958 tyrosinase. As this enzyme showed a higher diphenolase activity, a synthetic melanin from the enzymic oxidation of 3,4-dihydroxyphenylalanine (dopa) was obtained by the use of a Trametes versicolor (L.) Lloyd laccase for comparison. The natural and synthetic pigments were physico-chemically characterized by the use of ultraviolet (UV)-Visible, and Fourier transform infrared (FT-IR) and multifrequency electron paramagnetic resonance (EPR) spectroscopies. All the melanin samples displayed a stable free radical when analyzed by X-band EPR spectroscopy. Once the samples were recorded at Q-band EPR, a copolymer derived from a mixture of different constituents was evident in the natural melanin. All radical species were analyzed and discussed. The use of water-soluble melanin naturally produced by S. cyaneofuscatus Pridham et al. 1958 represents a new biotechnological alternative to commercial insoluble pigments.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje