Magnetic extraction and purification of DNA from whole human blood using a γ-Fe 2 O 3 @Chitosan@Polyaniline hybrid nanocomposite.

Autor: Maciel BG; Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil., da Silva RJ; Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil., Chávez-Guajardo AE; Unidad Académica de Ciencias de la Tierra, Universidad Autónoma de Zacatecas, 98058 Zacatecas, Zac, Mexico., Medina-Llamas JC; Centro de Estudios Científicos y Tecnológicos No. 18, Instituto Politécnico Nacional, 98160 Zacatecas, Zac, Mexico., Alcaraz-Espinoza JJ; Departamento de Física, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil., de Melo CP; Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Departamento de Física, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: celso@df.ufpe.br.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2018 Oct 01; Vol. 197, pp. 100-108. Date of Electronic Publication: 2018 May 22.
DOI: 10.1016/j.carbpol.2018.05.034
Abstrakt: We examined the properties of the nanocomposite γ-Fe 2 O 3 @Chi@Pani as an adsorbent of deoxyribonucleic acid (DNA). As a model system, we used an aqueous solution of salmon sperm DNA, whose decreasing concentration was followed by monitoring the 260 nm UV-vis absorption. After adjusting the data collected to a Langmuir isotherm curve, we estimated the adsorption capacity (q e ) of the nanocomposite as 49.5 mg/g. We also observed that the kinetic model of the DNA capture presents a mixed character, with both chemical mechanisms and intraparticle diffusion processes involved. When the MNC was used to extract the DNA from complex samples (human blood), a capture rate of 80 ng/μL was achieved, with the collected fraction exhibiting good quality, as evaluated by PCR analysis and electrophoresis assays. These results suggest that the γ-Fe 2 O 3 @Chi@Pani nanocomposite is a promising adsorbent for use in protocols for purification of DNA from complex samples.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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