| Autor: |
Ran T; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., Liu Y; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., Jiao JZ; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., Zhou CS; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., Tang SX; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., Wang M; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., He ZX; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., Tan ZL; 1Key Laboratory for Agro-Ecological Processes in Subtropical Region,and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,P.R. China., Yang WZ; 2Lethbridge Research Centre,Agriculture and Agri-Food Canada,Lethbridge,Alberta T1J 4B1,Canada., Beauchemin KA; 2Lethbridge Research Centre,Agriculture and Agri-Food Canada,Lethbridge,Alberta T1J 4B1,Canada. |
| Abstrakt: |
The gastrointestinal tract (GIT) of animals is capable of sensing various kinds of nutrients via G-protein coupled receptor-mediated signaling transduction pathways, and the process is known as 'gut nutrient chemosensing'. GPR40, GPR41, GPR43 and GPR119 are chemoreceptors for free fatty acids (FFAs) and lipid derivatives, but they are not well studied in small ruminants. The objective of this study is to determine the expression of GPR40, GPR41, GPR43 and GPR119 along the GIT of kid goats under supplemental feeding (S) v. grazing (G) during early development. In total, 44 kid goats (initial weight 1.35±0.12 kg) were slaughtered for sampling (rumen, abomasum, duodenum, jejunum, ileum, cecum, colon and rectum) between days 0 and 70. The expression of GPR41 and GPR43 were measured at both mRNA and protein levels, whereas GPR40 and GPR119 were assayed at protein level only. The effects of age and feeding system on their expression were variable depending upon GIT segments, chemoreceptors and expression level (mRNA or protein), and sometimes feeding system × age interactions (P0.05) on GPR43 expression; and there were no feeding system×age interactions (P>0.05) on GPR41 and GPR43 protein expression. The expression of GPR41 and GPR43 in rumen and abomasum linearly (P<0.01) increased with increasing age (from days 0 to 70). Meanwhile, age was the main factor affecting GPR40 expression throughout the GIT. These outcomes indicate that age and feeding system are the two factors affecting chemoreceptors for FFAs and lipid derivatives expression in the GIT of kids goats, and S enhanced the expression of chemoreceptors for FFAs, whereas G gave rise to greater expression of chemoreceptors for lipid derivatives. Our results suggest that enhanced expression of chemoreceptors for FFAs might be one of the benefits of early supplemental feeding offered to young ruminants during early development. |
