Rapid Detection of Listeria in Ice Cream in 13 Hours Using the Roka Listeria Detection Assay.

Autor: Arias-Rios EV; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Tenney K; IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Mai T; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Anderson S; IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Cantera RM; IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Nadala LM; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Han S; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Janagama H; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Pando JM; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Selover B; IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Lei Sung S; Microbiologique, Inc., 8215 Lake City Way NE, Seattle, WA 98115., Nadala C; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155., Samadpour M; Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155.
Jazyk: angličtina
Zdroj: Journal of AOAC International [J AOAC Int] 2018 Nov 01; Vol. 101 (6), pp. 1806-1812. Date of Electronic Publication: 2018 Jul 02.
DOI: 10.5740/jaoacint.18-0108
Abstrakt: Background: Listeria contamination is a major concern in the ice cream industry; therefore, early and accurate detection is vital. Current detection methods require about a 24 h enrichment period for detection. Objective: Enhance the early detection of Listeria in ice cream using the highly sensitive isothermal ribosomal RNA-based Roka/Atlas Listeria Detection Assay. Methods: The R2 Medium was developed for Listeria enrichment by Molecular Epidemiology, Inc. (Seattle, WA). Comparative growth curve studies were performed on the new R2 Medium for Listeria and the currently validated media for the Roka Listeria Detection Assay. Subsequently, a method comparison between the Roka Listeria Detection Assay and the U.S. Food and Drug Administration's (FDA) Bacteriological Analytical Manual (BAM) Chapter 10 reference method on ice cream was carried out. Results: The R2 Medium supports the growth of L. monocytogenes better than Buffered Listeria Enrichment Broth, Demi-Fraser broth, and Modified University of Vermont Broth, as indicated by the faster growth rate of the organism. When used as an enrichment medium in a method comparison study of ice cream, the results showed that R2 Medium-enriched samples tested with the Roka Listeria Detection Assay gave an equivalent performance compared with the 24 h FDA-BAM reference method at 10 and 18 h post-enrichment for Listeria . Conclusions: The results from this study indicate that the new R2 Medium and the highly sensitive Roka Listeria Detection Assay allowed for the rapid detection of Listeria species in ice cream in 13 h. Highlights: The Roka Listeria Detection Assay, in conjunction with a new media formulation (R2 Medium), allowed for the early detection of Listeria in ice cream and may be applied in other food matrixes and environmental samples.
Databáze: MEDLINE