Autor: |
Margesin R; 1Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria., Albuquerque L; 2Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal., Zhang DC; 3Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, PR China., Froufe HJC; 4Next Generation Sequencing Unit, Biocant, BiocantPark, Núcleo 04, Lote 8, 3060-197 Cantanhede, Portugal., Severino R; 4Next Generation Sequencing Unit, Biocant, BiocantPark, Núcleo 04, Lote 8, 3060-197 Cantanhede, Portugal., Roxo I; 4Next Generation Sequencing Unit, Biocant, BiocantPark, Núcleo 04, Lote 8, 3060-197 Cantanhede, Portugal., Egas C; 4Next Generation Sequencing Unit, Biocant, BiocantPark, Núcleo 04, Lote 8, 3060-197 Cantanhede, Portugal., da Costa MS; 2Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal. |
Abstrakt: |
A Gram-stain-negative, rod-shaped, motile, catalase and cytochrome c oxidase-positive bacterial strain, designated S20-91 T , was isolated from alpine forest soil. Growth occurred within a temperature range of 0-25 °C. Yeast extract was required for growth. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain S20-91 T was related to the genus Herminiimonas and had the highest 16S rRNA gene sequence similarity to Herminiimonas arsenicoxydans ULPAs1 T (96.5 %). The strain contained ubiquinone 8 as the predominant respiratory quinone and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. The major cellular fatty acids (>10 %) were C16 : 1ω7c (55.3 %) and C16 : 0 (25.6 %). The genomic DNA G+C content was 47.6 mol%. Combined data of genomic, phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-91 T represents a novel genus and species, for which the name Solimicrobium silvestre gen. nov., sp. nov. is proposed. The type strain is S20-91 T (=DSM 104733 T =LMG 30010). |