| Autor: |
Zurina IM; FSBSI 'Institute of General Pathology and Pathophysiology', Moscow, Russia. Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, Russia., Shpichka AI, Saburina IN, Kosheleva NV, Gorkun AA, Grebenik EA, Kuznetsova DS, Zhang D, Rochev YA, Butnaru DV, Zharikova TM, Istranova EV, Zhang Y, Istranov LP, Timashev PS |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical materials (Bristol, England) [Biomed Mater] 2018 Jul 19; Vol. 13 (5), pp. 054104. Date of Electronic Publication: 2018 Jul 19. |
| DOI: |
10.1088/1748-605X/aace1c |
| Abstrakt: |
Maintaining the epithelial status of cells in vitro and fabrication of a multilayered epithelial lining is one of the key problems in the therapy using cell technologies. When cultured in a monolayer, epithelial cells change their phenotype from epithelial to epithelial-mesenchymal or mesenchymal that makes it difficult to obtain a sufficient number of cells in a 2D culture and to use them in tissue engineering. Here, using buccal epithelial cells from the oral mucosa, we developed a novel approach to recover and maintain the stable cell phenotype and form a multilayered epithelial lining in vitro via the 2D/3D cell self-assembling. Transitioning the cells from the monolayer to non-adhesive 3D culture conditions led to formation of self-assembling spheroids, with restoration of their epithelial characteristics after epithelial-mesenchymal transition. In 7 days, the cells within spheroids restored the apical-basal polarity, and the formation of both tight (ZO1) and adherent (E-cadherin) intercellular junctions was shown. Thus, culturing buccal epithelial cells in a 3D system allowed us to recover and durably maintain the morphological and functional characteristics of epithelial cells. The multilayered epithelial lining formation was achieved after placing spheroids for 7 days onto a hybrid matrix, which consisted of collagen layers and reinforcing poly (lactide-co-glycolide) fibers and was proven promising for replacement of the urothelium. Thus, we offer an effective technique of forming multilayered epithelial linings on carrier-matrices using cell spheroids that was not previously described elsewhere and can find a wide range of applications in tissue engineering, replacement surgery, and regenerative medicine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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