Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides.
| Autor: | Brito FN; School of Medicine, Universidade Federal University do Pará, Belem, PA, Brazil., Vendramin FS; Surgery Division, UFPA, Belem, PA, Brazil., Lopes CTA; UFPA, Belem, PA, Brazil., Costa MPR; UFPA, Belem, PA, Brazil., Ohashi OM; Biomedicine Division, UFPA, Belem, PA, Brazil., Maia JGS; Chemistry Division, Universidade Federal do Oeste do Pará, Santarem, PA, Brazil., Ferreira LM; Plastic Surgery Division, Universidade Federal de São Paulo, Brazil., Silva JKDRD; Biotechnology Division, UFPA, Belem, PA, Brazil., Miranda MDS; Biotechnology Division, UFPA, Belem, PA, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Acta cirurgica brasileira [Acta Cir Bras] 2018 May; Vol. 33 (5), pp. 431-438. |
| DOI: | 10.1590/s0102-865020180050000005 |
| Abstrakt: | Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages. |
| Databáze: | MEDLINE |
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