| Autor: |
Weiss JM; Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute (NCI) at Frederick, Frederick, Maryland, USA., Davies LC; Cardiff University, Division of Infection and Immunity, Cardiff, United Kingdom., Karwan M; Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, Maryland, USA., Ileva L; Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, Maryland, USA., Ozaki MK; Women's Malignancies Branch, Center for Cancer Research (CCR), NCI, Bethesda, Maryland, USA., Cheng RY; Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute (NCI) at Frederick, Frederick, Maryland, USA., Ridnour LA; Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute (NCI) at Frederick, Frederick, Maryland, USA., Annunziata CM; Women's Malignancies Branch, Center for Cancer Research (CCR), NCI, Bethesda, Maryland, USA., Wink DA; Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute (NCI) at Frederick, Frederick, Maryland, USA., McVicar DW; Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute (NCI) at Frederick, Frederick, Maryland, USA. |
| Abstrakt: |
Control of cellular metabolism is critical for efficient cell function, although little is known about the interplay between cell subset-specific metabolites in situ, especially in the tumor setting. Here, we determined how a macrophage-specific (Mϕ-specific) metabolite, itaconic acid, can regulate tumor progression in the peritoneum. We show that peritoneal tumors (B16 melanoma or ID8 ovarian carcinoma) elicited a fatty acid oxidation-mediated increase in oxidative phosphorylation (OXPHOS) and glycolysis in peritoneal tissue-resident macrophages (pResMϕ). Unbiased metabolomics identified itaconic acid, the product of immune-responsive gene 1-mediated (Irg1-mediated) catabolism of mitochondrial cis-aconitate, among the most highly upregulated metabolites in pResMϕ of tumor-bearing mice. Administration of lentivirally encoded Irg1 shRNA significantly reduced peritoneal tumors. This resulted in reductions in OXPHOS and OXPHOS-driven production of ROS in pResMϕ and ROS-mediated MAPK activation in tumor cells. Our findings demonstrate that tumors profoundly alter pResMϕ metabolism, leading to the production of itaconic acid, which potentiates tumor growth. Monocytes isolated from ovarian carcinoma patients' ascites fluid expressed significantly elevated levels of IRG1. Therefore, IRG1 in pResMϕ represents a potential therapeutic target for peritoneal tumors. |
