Glutathione metabolism in type 2 diabetes and its relationship with microvascular complications and glycemia.

Autor: Lutchmansingh FK; Department of Basic Medical Sciences, The University of the West Indies, Mona, Jamaica., Hsu JW; Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, United States of America., Bennett FI; Department of Pathology, University Hospital of the West Indies; Mona, Jamaica., Badaloo AV; Caribbean Institute for Health Research, The University of the West Indies, Mona, Jamaica., McFarlane-Anderson N; Department of Basic Medical Sciences, The University of the West Indies, Mona, Jamaica., Gordon-Strachan GM; Health Research Unit, Faculty of Medical Sciences, The University of the West Indies, Mona, Jamaica., Wright-Pascoe RA; Department of Medicine, The University of the West Indies, Mona, Jamaica., Jahoor F; Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, United States of America., Boyne MS; Caribbean Institute for Health Research, The University of the West Indies, Mona, Jamaica.; Department of Medicine, The University of the West Indies, Mona, Jamaica.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Jun 07; Vol. 13 (6), pp. e0198626. Date of Electronic Publication: 2018 Jun 07 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0198626
Abstrakt: Aims/hypotheses: We hypothesized that there is decreased synthesis of glutathione (GSH) in type 2 diabetes (T2DM) especially in the presence of microvascular complications, and this is dependent on the degree of hyperglycemia.
Methods: In this case-control study, we recruited 16 patients with T2DM (7 without and 9 with microvascular complications), and 8 age- and sex-matched non-diabetic controls. We measured GSH synthesis rate using an infusion of [2H2]-glycine as isotopic tracer and collection of blood samples for liquid chromatography mass spectrometric analysis.
Results: Compared to the controls, T2DM patients had lower erythrocyte GSH concentrations (0.90 ± 0.42 vs. 0.35 ± 0.30 mmol/L; P = 0.001) and absolute synthesis rates (1.03 ± 0.55 vs. 0.50 ± 0.69 mmol/L/day; P = 0.01), but not fractional synthesis rates (114 ± 45 vs. 143 ± 82%/day; P = 0.07). The magnitudes of changes in patients with complications were greater for both GSH concentrations and absolute synthesis rates (P-values ≤ 0.01) compared to controls. There were no differences in GSH concentrations and synthesis rates between T2DM patients with and without complications (P-values > 0.1). Fasting glucose and HbA1c did not correlate with GSH concentration or synthesis rates (P-values > 0.17).
Conclusions: Compared to non-diabetic controls, patients with T2DM have glutathione deficiency, especially if they have microvascular complications. This is probably due to reduced synthesis and increased irreversible utilization by non-glycemic mechanisms.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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