d-galactose and aluminium chloride induced rat model with cognitive impairments.

Autor: Chiroma SM; Department of Human Anatomy, Faculty of Medicine and Health Sciences Universiti Putra Malaysia, Serdang Selangor, Malaysia; Department of Human Anatomy, College of Medical Sciences University of Maiduguri, Borno state, Nigeria., Mohd Moklas MA; Department of Human Anatomy, Faculty of Medicine and Health Sciences Universiti Putra Malaysia, Serdang Selangor, Malaysia. Electronic address: aris@upm.edu.my., Mat Taib CN; Department of Human Anatomy, Faculty of Medicine and Health Sciences Universiti Putra Malaysia, Serdang Selangor, Malaysia., Baharuldin MTH; Department of Human Anatomy, Faculty of Medicine and Health Sciences Universiti Putra Malaysia, Serdang Selangor, Malaysia., Amon Z; Faculty of Health Sciences UiTM Campus Puncak Alam, Puncak Alam Selangor, Malaysia.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Jul; Vol. 103, pp. 1602-1608. Date of Electronic Publication: 2018 May 07.
DOI: 10.1016/j.biopha.2018.04.152
Abstrakt: Cognitive impairments and cholinergic dysfunctions have been well reported in old age disorders including Alzheimer's disease (AD). d-galactose (D-gal) has been reported as a senescence agent while aluminium act as a neurotoxic metal, but little is known about their combined effects at different doses. The aim of this study was to establish an animal model with cognitive impairments by comparing the effects of different doses of co-administrated D-gal and aluminium chloride (AlCl 3 ). In this study male albino wistar rats were administered with D-gal 60 mg/kg.bwt intra peritoneally (I.P) injected and AlCl 3 (100, 200, or 300 mg/kg.bwt.) was orally administered once daily for 10 consecutive weeks. Performance of the rats were evaluated through behavioural assessments; Morris water maze (MWM) and open field tests (OFT); histopathological examination was performed on the hippocampus; moreover biochemical measurements of acetylcholinesterase (AChE) and hyperphosphorylated tau protein (p-tau) were examined. The results of this experiment on rats treated with D-gal 60 + AlCl 3 200 mg/kg.bwt showed near ideal cognitive impairments. The rats exhibited an obvious memory and learning deficits, marked neuronal loss in hippocampus, showed increase in AChE activities and high expression of p-tau within the tissues of the brain. This study concludes that D-gal 60 + AlCl 3 200 mg/kg.bwt as the ideal dose for mimicking AD like cognitive impairments in albino wistar rats. It is also crucial to understand the pathogenesis of this neurodegenerative disease and for drug discovery.
(Copyright © 2018 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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