Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects.

Autor: Vendruscolo CDP; Department of Clinical Medicine, School of Veterinary Medicine and Animals Science, University of São Paulo, São Paulo, São Paulo, Brazil., Moreira JJ; Department of Clinical Medicine, School of Veterinary Medicine and Animals Science, University of São Paulo, São Paulo, São Paulo, Brazil., Seidel SRT; Department of Clinical Medicine, School of Veterinary Medicine and Animals Science, University of São Paulo, São Paulo, São Paulo, Brazil., Fülber J; Department of Clinical Medicine, School of Veterinary Medicine and Animals Science, University of São Paulo, São Paulo, São Paulo, Brazil., Neuenschwander HM; Department of Clinical Medicine, School of Veterinary Medicine and Animals Science, University of São Paulo, São Paulo, São Paulo, Brazil., Bonagura G; Department of Large Animals Clinics, Anhembi Morumbi University, São Paulo, São Paulo, Brazil., Agreste FR; Department of Clinical Medicine, School of Veterinary Medicine and Animals Science, University of São Paulo, São Paulo, São Paulo, Brazil., Baccarin RYA; Department of Clinical Medicine, School of Veterinary Medicine and Animals Science, University of São Paulo, São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 May 29; Vol. 13 (5), pp. e0197736. Date of Electronic Publication: 2018 May 29 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0197736
Abstrakt: Objective: The aim of this study was to verify whether transient inflammatory reactions induced by intra-articular medicinal ozone administration affect joint components, by in vivo evaluation of inflammatory (prostaglandin E2, Substance P, Interleukin-6, Interleukine-1, Tumor Necrosis Factor), anti-inflammatory (Interleukin-10) and oxidative (superoxide dismutase activity and oxidative burst) biomarkers and extracellular matrix degradation products (chondroitin sulphate and hyaluronic acid) in synovial fluid.
Methods: The effects of medicinal ozone were analyzed at two ozone concentrations (groups A and B, 20 and 40 μg/ml, respectively), using oxygen-injected joints as controls (group C); each group received ten treatments (15 ml gas per treatment). Physical evaluation, evaluation of lameness, ultrasonography, and synovial fluid analysis were performed.
Results: All joints presented mild and transient effusion throughout the study. Group B exhibited the highest lameness score on day 14 (P<0.05), detected by the lameness measurement system, probably because of the higher ozone concentration. All groups exhibited increased ultrasonography scores on day 14 (P < 0.05). Groups A and B exhibited increased proteins concentrations on day 21 (P<0.05). There was no change in hyaluronic acid concentration or the percentage of high-molecular weight hyaluronic acid throughout the experiment. Chondroitin sulfate concentrations decreased in group B, and did not change in group A and C, indicating that neither treatment provoked extracellular matrix catabolism. Cytokine and eicosanoid concentrations were not significantly changed.
Conclusions: The ozonetherapy did not cause significant inflammation process or cartilage degradation, therefore, ozonetherapy is safe at both evaluated doses.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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