Monoclonal Antibody DL11C8 Identifies ADAM23 as a Component of Lipid Raft Microdomains.

Autor: Borgonovo ZLM; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil; Department of Cell Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Ribeiro CF; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Costa MDM; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil; Department of Structural and Molecular Biology and Genetics, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil., Souza ILM; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil; Department of Cell Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Rossi GR; Department of Cell Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Alcantara MV; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Ingberman M; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Braga LG; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Mercadante AF; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Nakao LS; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil; Department of Cell Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Zanata SM; Department of Basic Pathology, Universidade Federal do Paraná, Curitiba, PR, Brazil; Department of Cell Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil. Electronic address: smzanata@ufpr.br.
Jazyk: angličtina
Zdroj: Neuroscience [Neuroscience] 2018 Aug 01; Vol. 384, pp. 165-177. Date of Electronic Publication: 2018 May 22.
DOI: 10.1016/j.neuroscience.2018.05.016
Abstrakt: A disintegrin and metalloprotease protein 23 (ADAM23) is a transmembrane type I glycoprotein involved with the development and maintenance of the nervous system, including neurite outgrowth, neuronal adhesion and differentiation and regulation of synaptic transmission. In addition, ADAM23 seems to participate in immune response and tumor establishment through interaction with different members of integrin receptors. Here, we describe a novel monoclonal antibody (DL11C8) that specifically recognizes the cysteine-rich domain of both pre-protein (100 kDa) and mature (70 kDa) forms of ADAM23 from different species, including human, rodents and avian orthologs. Using this antibody, we detected both forms of ADAM23 on the cell surface of three neuronal cell lineages (Neuro-2a, SH-SY5Y and CHLA-20), with a higher relative content of ADAM23 100 kDa . Furthermore, we demonstrate for the first time that a catalytically inactive member of the ADAM family is present in the membrane signaling platforms, namely lipid rafts. Indeed, the mature ADAM23 70 kDa partitions between raft and non-raft membrane domains, while the pro-protein ADAM23 100 kDa is mainly expressed in non-raft domains. These membranous distributions were observed in both different brain regions homogenates and primary cultured neurons lysates from mouse cortex and cerebellum. Taken together, these findings point out ADAM23 as a lipid raft molecular component.
(Copyright © 2018 IBRO. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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