A new method for determining levels of sedation in dogs: A pilot study with propofol and a novel neuroactive steroid anesthetic.

Autor: Youngblood BL; QTest Laboratories, 6456 Fiesta Drive, Columbus, OH 43235, USA. Electronic address: byoungblood@qtestlabs.com., Ueyama Y; QTest Laboratories, 6456 Fiesta Drive, Columbus, OH 43235, USA., Muir WW; QTest Laboratories, 6456 Fiesta Drive, Columbus, OH 43235, USA., Belfort GM; Sage Therapeutics, 215 First Street, Cambridge, MA 02142, USA., Hammond RH; Sage Therapeutics, 215 First Street, Cambridge, MA 02142, USA., Dai J; Sage Therapeutics, 215 First Street, Cambridge, MA 02142, USA., Salituro FG; Sage Therapeutics, 215 First Street, Cambridge, MA 02142, USA., Robichaud AJ; Sage Therapeutics, 215 First Street, Cambridge, MA 02142, USA., Doherty JJ; Sage Therapeutics, 215 First Street, Cambridge, MA 02142, USA.
Jazyk: angličtina
Zdroj: Journal of neuroscience methods [J Neurosci Methods] 2018 Jul 15; Vol. 305, pp. 82-88. Date of Electronic Publication: 2018 May 23.
DOI: 10.1016/j.jneumeth.2018.05.006
Abstrakt: Background: Different levels of consciousness are required in order to perform different medical procedures. Sedation scales established to objectively define various levels of sedation in humans have not been thoroughly characterized in non-human species. Postural changes in rats or dogs are useful as gross measures of sedation but are inadequate for quantitative assessment since graded levels of sedation are difficult to delineate and obscured by movement abnormalities.
New Method: A new canine sedation scoring (CSS) method was developed based on the modified observer's assessment of alertness and sedation score (MOAA/S) used in humans. The method employed a combination of physical, auditory and somatosensory stimuli of increasing intensity. Cardiovascular, respiratory, and a neurophysiological measure of sedation (bispectral index: BIS) data were recorded. Validation studies were performed following intravenous loading and constant rate infusion of propofol or a novel synthetic neuroactive steroid (SGE-746).
Results: Four levels of consciousness were identified: 1) Awake, 2) Moderate Sedation (MS), 3) Deep Sedation (DS) and 4) General Anesthesia (GA). Cardiorespiratory measurements obtained after bolus administration of propofol and SGE-746 and at the end of each CRI remained within normal limits. Canine sedation scores correlated with BIS for SGE-746. SGE-746 exhibited a more gradual exposure-response relationship than propofol. Larger increases in the plasma concentration from awake values were required to achieve different levels of sedation with SGE-746 compared to propofol.
Comparison With Existing Methods: No other canine sedation scoring methods are widely accepted.
Conclusion: A CSS method, based on the human MOAA/S scale defined four levels of consciousness in dogs and provided better resolution of sedation depth than BIS alone.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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