Iron-specific Signal Separation from within Heavy Metal Stained Biological Samples Using X-Ray Microtomography with Polychromatic Source and Energy-Integrating Detectors.

Autor:	Katchalski T; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA. tkatchalski@ucsd.edu., Case T; Carl Zeiss X-Ray Microscopy, 4385 Hopyard Road, Suite 100, Pleasanton, CA, 94588, USA., Kim KY; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Ramachandra R; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Bushong EA; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Deerinck TJ; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Haberl MG; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Mackey MR; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Peltier S; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Castillon GA; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Fujikawa N; Carl Zeiss X-Ray Microscopy, 4385 Hopyard Road, Suite 100, Pleasanton, CA, 94588, USA., Lawrence AR; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA., Ellisman MH; National Center for Microscopy and Imaging Research (NCMIR), University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA. mellisman@ucsd.edu.; Departments of Neurosciences and Bioengineering, University of California San Diego, 9500 Gilman Dr. MC 0608, La Jolla, CA, 92093-0608, USA. mellisman@ucsd.edu.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2018 May 15; Vol. 8 (1), pp. 7553. Date of Electronic Publication: 2018 May 15.
DOI:	10.1038/s41598-018-25099-z
Abstrakt:	Biological samples are frequently stained with heavy metals in preparation for examining the macro, micro and ultra-structure using X-ray microtomography and electron microscopy. A single X-ray microtomography scan reveals detailed 3D structure based on staining density, yet it lacks both material composition and functional information. Using a commercially available polychromatic X-ray source, energy integrating detectors and a two-scan configuration labelled by their energy- "High" and "Low", we demonstrate how a specific element, here shown with iron, can be detected from a mixture with other heavy metals. With proper selection of scan configuration, achieving strong overlap of source characteristic emission lines and iron K-edge absorption, iron absorption was enhanced enabling K-edge imaging. Specifically, iron images were obtained by scatter plot material analysis, after selecting specific regions within scatter plots generated from the "High" and "Low" scans. Using this method, we identified iron rich regions associated with an iron staining reaction that marks the nodes of Ranvier along nerve axons within mouse spinal roots, also stained with osmium metal commonly used for electron microscopy.
Databáze:	MEDLINE
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