| Autor: |
Grebenik EA; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, 8-2 Trubetskaya st., Moscow, 119991, Russia., Grinchenko VD, Churbanov SN, Minaev NV, Shavkuta BS, Melnikov PA, Butnaru DV, Rochev YA, Bagratashvili VN, Timashev PS |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical materials (Bristol, England) [Biomed Mater] 2018 Jun 06; Vol. 13 (5), pp. 054103. Date of Electronic Publication: 2018 Jun 06. |
| DOI: |
10.1088/1748-605X/aac4cb |
| Abstrakt: |
This study was aimed to design and characterise hybrid tissue-engineered constructs composed of osteoinducing polylactide-based scaffolds with multi-layered cellular biointerface for bone tissue reconstruction. Three-dimensional scaffolds with improved hydrophilic and osteoinducing properties were produced using the surface-selective laser sintering (SSLS) method. The designed scaffold pattern had dimensions of 8 × 8 × 2.5 mm and ladder-like pores (∼700 μm in width). Hyaluronic acid-coated polylactide microparticles (∼100 μm in diameter) were used as building blocks and water was used as the photosensitizer for SSLS followed by photocross-linking with Irgacure 2959 photoinitiator. Resulting scaffolds provided successful adhesion and expansion of human bone marrow mesenchymal stromal cells from a single-cell suspension. Induced calcium deposition by the cells associated with osteogenic differentiation was detected in 7-21 days of culturing in basal medium. The values were up to 60% higher on scaffolds produced at a higher prototyping speed under the experimental conditions. Innovative approach to graft the scaffolds with multi-layered cell sheets was proposed aiming to facilitate host tissue-implant integration. The sheets of murine MS-5 stromal cell line exhibited contiguous morphology and high viability in a modelled construct. Thus, the SSLS method proved to be effective in designing osteoinducing scaffolds suitable for the delivery of cell sheets. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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