Evaluation of thrombin inhibitory activity of catechins by online capillary electrophoresis-based immobilized enzyme microreactor and molecular docking.
Autor: | Li QQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China., Yang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China. Electronic address: fengqingyang@cqu.edu.cn., Wang YZ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China., Wu ZY; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China., Xia ZN; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China., Chen H; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China. |
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Jazyk: | angličtina |
Zdroj: | Talanta [Talanta] 2018 Aug 01; Vol. 185, pp. 16-22. Date of Electronic Publication: 2018 Mar 18. |
DOI: | 10.1016/j.talanta.2018.03.049 |
Abstrakt: | An online capillary electrophoresis (CE)-based thrombin (THR) immobilized enzyme microreactor (IMER) method was established to screen THR inhibitors in this study. S-2366 was used as chromogenic substrate for determination of THR activity and other kinetic constants. After continuously run for 50 times, the prepared IMER could still remain 89% of the initial immobilized enzyme activity. The Michaelis-Menten constant (K (Copyright © 2018 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
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