| Autor: |
Dos Santos Sales V; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Monteiro ÁB; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Delmondes GA; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., do Nascimento EP; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Sobreira Dantas Nóbrega de Figuêiredo FR; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., de Souza Rodrigues CK; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Evangelista de Lacerda JF; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Fernandes CN; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Barbosa MO; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Brasil AX; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Tintino SR; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Vega Gomez MC; Center for the Development of Scientific Research, Foundation Moisés Bertoni/Laboratories Diaz Gill, Asunción-Paraguay., Coronel C; Center for the Development of Scientific Research, Foundation Moisés Bertoni/Laboratories Diaz Gill, Asunción-Paraguay., Melo Coutinho HD; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Martins da Costa JG; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Bezerra Felipe CF; Department of Molecular Biology, Federal University of Paraíba, Paraíba, PB, Brazil., Alencar de Menezes IR; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Kerntopf MR; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil. |
| Abstrakt: |
With the increase of neglected diseases such as leishmaniasis and Chagas disease, there was a need for the search for new therapeutic alternatives that reduce the harm caused by medicine available for treatment. Thus, this study was performed to investigate the antiparasitic activity of the essential oil from the fruits of Piper tuberculatum Jacq, against lines of Leishmania braziliensis (MHOM/CO/88/UA301), Leishmania infantum (MHOM/ES/92/BCN83) and Trypanosoma cruzi (LC-B5 clone). Before running protocols, an analysis of the chemical composition of essential oil was conducted, which presented monoterpenes and sesquiterpenes. As major constituents, β-pinene and α-pinene were identified. Regarding to antiparasitic activity, the essential oil had an EC 50 values of 133.97 µg/mL and 143.59 µg/mL against variations promastigotes of L. infantum and L. braziliensis , respectively. As for trypanocidal activity, the oil showed EC 50 value of 140.31 µg/mL against epimastigote form of T. cruzi . Moreover, it showed moderate cytotoxicity in fibroblasts with LC 50 value of 204.71 µg/mL. The observed effect may be related to the presence of terpenes contained in the essential oil, since it has its antiparasitic activity proven in the literature. |
