Cell-sized lipid vesicles for cell-cell synaptic therapies.

Autor:	Vallejo D; Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science Il, Irvine, California 92697, USA., Lee SH; Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science Il, Irvine, California 92697, USA., Lee D; School of Medicine, University of California at Irvine, 1001 Health Sciences Rd, Irvine, CA, 92617, USA., Zhang C; School of Medicine, University of California at Irvine, 1001 Health Sciences Rd, Irvine, CA, 92617, USA., Rapier C; Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science Il, Irvine, California 92697, USA., Chessler SD; School of Medicine, University of California at Irvine, 1001 Health Sciences Rd, Irvine, CA, 92617, USA., Lee AP; Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science Il, Irvine, California 92697, USA.; Department of Mechanical and Aerospace Engineering, University of California at Irvine, 3120 Natural Science Il, Irvine, California 92697, USA.
Jazyk:	angličtina
Zdroj:	Technology [Technology (Singap World Sci)] 2017 Dec; Vol. 5 (4), pp. 201-213. Date of Electronic Publication: 2018 Jan 24.
DOI:	10.1142/S233954781750011X
Abstrakt:	Cell-sized lipid vesicles (CLVs) have shown great promise for therapeutic and artificial cell applications, but their fragility and short shelf life has hindered widespread adoption and commercial viability. We present a method to circumvent the storage limitations of CLVs such as giant unilamellar vesicles (GUVs) and single-compartment multisomes (SCMs) by storing them in a double emulsion precursor form. The double emulsions can be stored for at least 8 months and readily converted into either GUVs or SCMs at any time. In this study, we investigate the interfacial parameters responsible for this morphological change, and we also demonstrate the therapeutic potential of CLVs by utilizing them to present a transmembrane protein, neuroligin-2, to pancreatic β-cells, forming cell-cell synapses that stimulate insulin secretion and cellular growth. Competing Interests: CONFLICTS OF INTEREST There are no conflicts to declare.
Databáze:	MEDLINE
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