| Autor: |
Abdel Gawad SS; Parasitology Department, Faculty of Medicine, Beni-Suef University, Egypt., Ismail MAM; Parasitology Department, Faculty of Medicine, Cairo University, Egypt., Imam NFA; Parasitology Department, Faculty of Medicine, Cairo University, Egypt., Eassa AHA; Parasitology Department, Faculty of Medicine, Cairo University, Egypt., Abu-Sarea EY; Parasitology Department, Faculty of Medicine, Beni-Suef University, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
The Korean journal of parasitology [Korean J Parasitol] 2018 Apr; Vol. 56 (2), pp. 113-119. Date of Electronic Publication: 2018 Apr 30. |
| DOI: |
10.3347/kjp.2018.56.2.113 |
| Abstrakt: |
Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20-40 year-old group, followed by 40-60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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