Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask.

Autor:	Kieninger J; Laboratory for Sensors, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, Germany. kieninger@imtek.uni-freiburg.de., Tamari Y; Laboratory for Sensors, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, Germany., Enderle B; Laboratory for Sensors, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, Germany. barbara.enderle@imtek.uni-freiburg.de., Jobst G; Jobst Technologies GmbH, Engesserstraße 4b, D-79108 Freiburg, Germany. gj@jobst-technologies.com., Sandvik JA; Department of Physics, University of Oslo, 1048 Blindern, N-0316 Oslo, Norway. j.a.sandvik@fys.uio.no., Pettersen EO; Department of Physics, University of Oslo, 1048 Blindern, N-0316 Oslo, Norway. e.o.pettersen@fys.uio.no., Urban GA; Laboratory for Sensors, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, Germany. urban@imtek.de.
Jazyk:	angličtina
Zdroj:	Biosensors [Biosensors (Basel)] 2018 Apr 26; Vol. 8 (2). Date of Electronic Publication: 2018 Apr 26.
DOI:	10.3390/bios8020044
Abstrakt:	The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be equipped with any electrochemical sensor. Its transparency allows optical inspection of the cells during measurement. The surface of the sensor chip is in-plane with the flask surface allowing undisturbed cell growth on the sensor chip. A custom developed rack system allows easy usage of multiple flasks in parallel within an incubator. The presented data demonstrates the application of the SCCF with brain tumor (T98G) and breast cancer (T-47D) cells. Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular acidification was accessed with potentiometric pH sensors using electrodeposited iridium oxide films. The system itself provides the foundation for electrochemical monitoring systems in 3D cell culture.
Databáze:	MEDLINE
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