Aflatoxin B 1 -contaminated diet disrupts the blood-brain barrier and affects fish behavior: Involvement of neurotransmitters in brain synaptosomes.

Autor: Baldissera MD; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: mdbaldissera@outlook.com., Souza CF; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Zeppenfeld CC; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Descovi SN; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Moreira KLS; Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., da Rocha MIUM; Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., da Veiga ML; Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., da Silva AS; Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, RS, Brazil., Baldisserotto B; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: bbaldisserotto@hotmail.com.
Jazyk: angličtina
Zdroj: Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2018 Jun; Vol. 60, pp. 45-51. Date of Electronic Publication: 2018 Apr 04.
DOI: 10.1016/j.etap.2018.04.003
Abstrakt: It is known that the cytotoxic effects of aflatoxin B 1 (AFB 1 ) in endothelial cells of the blood-brain barrier (BBB) are associated with behavioral dysfunction. However, the effects of a diet contaminated with AFB 1 on the behavior of silver catfish remain unknown. Thus, the aim of this study was to evaluate whether an AFB 1 -contaminated diet (1177 ppb kg feed -1 ) impaired silver catfish behavior, as well as whether disruption of the BBB and alteration of neurotransmitters in brain synaptosomes are involved. Fish fed a diet contaminated with AFB 1 presented a behavioral impairment linked with hyperlocomotion on days 14 and 21 compared with the control group (basal diet). Neurotransmitter levels were also affected on days 14 and 21. The permeability of the BBB to Evans blue dye increased in the intoxicated animals compared with the control group, which suggests that the BBB was disrupted. Moreover, acetylcholinesterase (AChE) activity in brain synaptosomes was increased in fish fed a diet contaminated with AFB 1 , while activity of the sodium-potassium pump (Na + , K + -ATPase) was decreased. Based on this evidence, the present study shows that silver catfish fed a diet containing AFB 1 exhibit behavioral impairments related to hyperlocomotion. This diet caused a disruption of the BBB and brain lesions, which may contribute to the behavioral changes. Also, the alterations in the activities of AChE and Na + , K + -ATPase in brain synaptosomes may directly contribute to this behavior, since they may promote synapse dysfunction. In addition, the hyperlocomotion may be considered an important macroscopic marker indicating possible AFB 1 intoxication.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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